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Anti-IRF6 Antibody (aa455-467) IHC-plus™ LS-B3231

Note: This antibody replaces LS-C55013

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IRF6 Antibodies

Anti-IRF6 Antibody (aa455-467) IHC-plus™ LS-B3231
Goat Polyclonal to Human IRF6
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IRF6 Antibody (aa395-444) IHC-plus™ LS-B6678
Rabbit Polyclonal to Human IRF6
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Sheep, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Goat Polyclonal to Human IRF6
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human IRF6
Goat
Human (tested or 100% immunogen sequence identity)
Monkey, Horse (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • ELISA

Specificity and Use

IRF6 antibody was raised against synthetic peptide C-TPSMQLPPALPPQ from the C-terminus of human IRF6 (NP_006138.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset, Horse (92%); Rat, Hamster, Elephant, Panda, Dog, Bat, Pig (85%).
LS-E25340 - Lyophilized - 100 µg - $145.00
aa455-467
Human IRF6.
Immunohistochemistry: LS-B3231 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3231 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

Publications (1)

1
Hailey-Hailey disease: investigation of a possible compensatory SERCA2 up-regulation and analysis of SPCA1, p63, and IRF6 expression. Zhang D, Li X, Wang Z, Zhang Y, Guo K, Wang S, Tu C, Huo J, Xiao S. Archives of dermatological research. Mar;307:143-9. [Full Text Article] [PubMed:25256005]

About IRF6

O14896 NM_006147 NP_006138.1

IRF6 Antibody, Interferon regulatory factor 6 Antibody, OFC6 Antibody, PPS1 Antibody, VWS Antibody, VWS1 Antibody, IRF-6 Antibody, LPS Antibody, PIT Antibody, PPS Antibody, Van der Woude syndrome Antibody

IRF6 is a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6.

Immunohistochemistry

Immunohistochemistry
Anti-IRF6 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3231 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-IRF6 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3231 concentration 5 ug/ml.

Western blot

Western blot
Antibody staining (0.2 ug/ml) of Human Ovary lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
 

Requested From: United States
Date Requested: 12/4/2016

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