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Anti-IRF2BP1 Antibody (aa178-227) LS-C135339

Catalog Size Price
LS-C135339-50 50 µg Unavailable

Related Products

16 IRF2BP1 Antibodies

Most Popular IRF2BP1 Antibodies

Anti-IRF2BP1 Antibody (aa468-517) LS-C102613
Rabbit Polyclonal (IgG) to Human IRF2BP1
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Western blot
Unconjugated
IRF2BP1 antibody LS-C102613 Western blot of Placenta lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-IRF2BP1 Antibody (aa109-122) LS-C139734
Goat Polyclonal to Human IRF2BP1
Human, Monkey, Bovine, Dog, Horse, Pig
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
IRF2BP1 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-IRF2BP1 Antibody (aa461-474) LS-C139735
Goat Polyclonal to Human IRF2BP1
Human, Monkey, Rat
Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
IRF2BP1 antibody (0.5 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-IRF2BP1 Antibody (aa450-500) LS-C289884
Rabbit Polyclonal (IgG) to Human IRF2BP1
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Western blot, Immunoprecipitation
Unconjugated
Detection of Human IRF2BP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-IRF2BP1 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. IRF2BP1 was also immunoprecipitated by rabbit anti-IRF2BP1 antibody BL11357, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Anti-IRF2BP1 Antibody LS-C666491
Rabbit Polyclonal (IgG) to Human IRF2BP1
Human
IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

100% Guaranteed
Rabbit Polyclonal to Rat IRF2BP1
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Rat IRF2BP1
Rabbit
Rat, Human, Monkey, Mouse, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

IRF2BP1 antibody was raised against synthetic peptide located between aa178-227 of rat Irf2bp1 (D4AAZ8, NP_001100953). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%); Galago (92%).
LS-E19975 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135339. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa178-227
Rat IRF2BP1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About IRF2BP1

Q8IU81 AL080159 Q8IU81

IRF2BP1 Antibody, DKFZP434M154 Antibody, IRF-2-binding protein 1 Antibody, IRF-2BP1 Antibody

Acts as a transcriptional corepressor in a IRF2-dependent manner; this repression is not mediated by histone deacetylase activities. May act as an E3 ligase towards JDP2, enhancing its polyubiquitination. Represses ATF2-dependent transcriptional activation.

Western blot

IRF2BP1 antibody LS-C135339 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IRF2BP1 antibody LS-C135339 Western blot of Rat Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/26/2018

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