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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IRF2BP1 Antibody (aa109‑122) LS‑C139734
IRF2BP1 antibody LS-C139734 is an unconjugated goat polyclonal antibody to IRF2BP1 from human, bovine, dog and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C139734-100
100 µg
Unavailable

Popular IRF2BP1 Products

IRF2BP1 antibody LS-C102613 Western blot of Placenta lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: Western blot
IRF2BP1 antibody (0.5 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Monkey, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Detection of Human IRF2BP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-IRF2BP1 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. IRF2BP1 was also immunoprecipitated by rabbit anti-IRF2BP1 antibody BL11357, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Rabbit
Applications: Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA

Product Description

IRF2BP1 antibody LS-C139734 is an unconjugated goat polyclonal antibody to IRF2BP1 from human, bovine, dog and other species. Validated for Peptide-ELISA and WB.
About IRF2BP1
Acts as a transcriptional corepressor in a IRF2-dependent manner; this repression is not mediated by histone deacetylase activities. May act as an E3 ligase towards JDP2, enhancing its polyubiquitination. Represses ATF2-dependent transcriptional activation. Q8IU81 AL080159 Q8IU81

IRF2BP1 Antibody, DKFZP434M154 Antibody, IRF-2-binding protein 1 Antibody, IRF-2BP1 Antibody


Specifications

Target
Human IRF2BP1
Host
Goat
Reactivity
Human, Monkey, Bovine, Dog, Horse, Pig (tested or 100% immunogen sequence identity)
Predicted
Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Immunogen
IRF2BP1 antibody was raised against synthetic peptide C-QDRYDRATSSGRLP from an internal region of human IRF2BP1 (NP_056464.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Pig (100%); Rabbit (93%).
Blocking Peptide
LS-E27601 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139734. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa109-122
Specificity
Human IRF2BP1.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 75kD band observed in lysates of cell line HepG2 (calculated MW of 61.7kD according to NP_056464.1). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

IRF2BP1 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IRF2BP1 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

IRF2BP1 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IRF2BP1 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

IRF2BP1 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IRF2BP1 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

IRF2BP1 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
IRF2BP1 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/25/2018

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