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Anti-IRAK4 / IRAK-4 Antibody (clone 2H9) IHC-plus™ LS-B10452

Note: This antibody replaces LS-C169398
See our new IRAK4 / IRAK-4 ELISA Kits
Human IRAK4 / IRAK-4 ELISA Kit - LS-F4782
Rat IRAK4 / IRAK-4 ELISA Kit - LS-F8523
(kits do not necessarily contain the antibody listed on this page)

Ordering

Wt. Vol. Conc. Price
- 100 µl - $395

LSBio (Direct) LSBio (Direct)
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IRAK4 / IRAK-4 Antibody LS-B10452 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-IRAK4 / IRAK-4 antibody IHC staining of human small intestine.
Western blot
Western blot using IRAK4 monoclonal antibody against human IRAK4 (AA: 21-198) recombinant protein.
Western blot
Western blot using IRAK4 mouse monoclonal antibody against THP-1 (1), HeLa (2), K562 (3), MCF-7...
Enzyme-Linked Immunosorbent Assay
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

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Mouse Monoclonal [clone 2H9] (IgG1) to Human IRAK4 / IRAK-4
Human, Monkey, Mouse
IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human IRAK4 / IRAK-4
Mouse
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2H9]
Ascites

Applications

IHC (1:200 - 1:1000), IHC - Paraffin (1:50), Western blot (1:500 - 1:2000), Flow Cytometry (1:200 - 1:400), ELISA (1:10000)

Specificity and Use

IRAK4 / IRAK-4 antibody was raised against purified recombinant fragment of human IRAK4 expressed in E. coli.
Human IRAK4

Packaging

Ascites, 0.03% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About IRAK4 / IRAK-4

Q9NWZ3 NM_016123 NP_057207.2

IRAK4 Antibody, IPD1 Antibody, IRAK-4 Antibody, NY-REN-64 Antibody, REN64 Antibody

IRAK4, an IRAK type protein kinase, is a signal transducer for the immune response Toll-like receptor and interleukin-1 (IL-1) receptor signaling cascades. IRAK4 has auto- and cross-phosphorylation kinase activity and has been shown to phosphorylate and activate IRAK1. Additionally, IRAK4 interacts with IRAK1 and TRAF6 in an IL-1-dependent manner, and overexpression of IRAK4 can activate NF-kappaB as well as mitogen-activated protein (MAP) kinase pathways.

Immunohistochemistry

Immunohistochemistry
Anti-IRAK4 / IRAK-4 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10452 dilution 1:50.

Western blot

Western blot
Western blot using IRAK4 monoclonal antibody against human IRAK4 (AA: 21-198) recombinant protein. (Expected MW is 45.4 kDa)

Western blot

Western blot
Western blot using IRAK4 mouse monoclonal antibody against THP-1 (1), HeLa (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: United States
Date Requested: 4/18/2015

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