LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
IRAK4 / IRAK‑4 Antibody (aa436‑459) LS‑C8256
IRAK-4 antibody LS-C8256 is an unconjugated rabbit polyclonal antibody to human IRAK-4 (IRAK4). Validated for WB.
Catalog
Size
Price
LS-C8256-100
100 µg
$575
Description
IRAK-4 antibody LS-C8256 is an unconjugated rabbit polyclonal antibody to human IRAK-4 (IRAK4). Validated for WB.
Target
Human IRAK4 / IRAK-4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (0.5 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
IRAK4 / IRAK-4 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 436-459 (CLHEKKNKRPDIKKVQQLLQEMTA) of human IRAK4. The immunizing sequence has 21/24 identical amino acids in mouse. Percent identity by BLAST analysis: Human, Gibbon, Marmoset, Porcine (100%); Gorilla, Monkey, Elephant, Horse (96%); Bovine, Rabbit, Platypus (92%); Mouse, Hamster, Bat (88%); Rat (83%).
Epitope
aa436-459
Specificity
Recognizes IRAK4, Mr ~52kD. Species cross-reactivity: Human. Predicted to cross-react with mouse based on sequence homology.
Usage
Suitable for use in Western Blot. Western Blot: 0.5 ug/ml detects IRAK4 in RIPA lysates from HeLa and A431 cells. HeLa cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-IRAK4 (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About IRAK4 / IRAK-4
Q9NWZ3 NM_016123 NP_057207.2

Popular IRAK4 / IRAK-4 Products

Anti-IRAK4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Mouse, Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-IRAK4 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Species: Human, Monkey, Bat, Horse
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-IRAK4 / IRAK-4 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Anti-IRAK4 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: Western blot, ELISA

Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 11/20/2018
Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy