IRAK-4 antibody LS-C8256 is an unconjugated rabbit polyclonal antibody to human IRAK-4 (IRAK4). Validated for WB.
Human IRAK4 / IRAK-4
(tested or 100% immunogen sequence identity)
Monkey, Bovine, Horse, Rabbit (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (0.5 µg/ml)
- (applications tested for the base form of this product only)
IRAK4 / IRAK-4 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 436-459 (CLHEKKNKRPDIKKVQQLLQEMTA) of human IRAK4. The immunizing sequence has 21/24 identical amino acids in mouse. Percent identity by BLAST analysis: Human, Gibbon, Marmoset, Porcine (100%); Gorilla, Monkey, Elephant, Horse (96%); Bovine, Rabbit, Platypus (92%); Mouse, Hamster, Bat (88%); Rat (83%).
Recognizes IRAK4, Mr ~52kD. Species cross-reactivity: Human. Predicted to cross-react with mouse based on sequence homology.
Suitable for use in Western Blot. Western Blot: 0.5 ug/ml detects IRAK4 in RIPA lysates from HeLa and A431 cells. HeLa cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-IRAK4 (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.