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Anti-IRAK2 / IRAK-2 Antibody (C-Terminus) IHC-plus™ LS-B3201

Note: This antibody replaces LS-C487
Catalog Size Price
LS-B3201-50 50 µg (1 mg/ml) Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human IRAK2 / IRAK-2
Human
IHC, IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated

Details

Human IRAK2 / IRAK-2
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (0.5 - 1 µg/ml)
  • ELISA

Specificity and Use

LS-E30169 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B3201. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
C-Terminus
At least four isoforms of IRAK2 are known to exist; this antibody will detect all four isoforms. Anti-IRAK2 has no cross response to IRAK.
Immunohistochemistry: LS-B3201 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3201 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Store at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze thaw cycles.
For research use only.

About IRAK2 / IRAK-2

O43187 NM_001570 NP_001561.3

IRAK2 Antibody, Il1rak2 Antibody, IRAK-2 Antibody

IRAK2, an IRAK type protein kinase, is a signal transducer for the immune response interleukin-1 receptor (IL-1R) signaling cascade. IRAK2 also has been associated with signaling cell death, potentially via the Fas-associated death domain protein/caspase-8 apoptotic pathway. Dominant negative forms of IRAK2 attenuate IL-1R-mediated NF-kappa B activation, suggesting that IRAK-2 may serve as a therapeutic target for inhibiting IL-1-induced inflammation.

Immunohistochemistry

Anti-IRAK2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3201 concentration 5 ug/ml.
Anti-IRAK2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3201 concentration 5 ug/ml.

Immunohistochemistry

Anti-IRAK2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3201 concentration 5 ug/ml.
Anti-IRAK2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3201 concentration 5 ug/ml.

Immunohistochemistry

Anti-IRAK2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3201 concentration 5 ug/ml.
Anti-IRAK2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3201 concentration 5 ug/ml.

Immunohistochemistry

Anti-IRAK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3201 concentration 5 ug/ml.
Anti-IRAK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3201 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of mIRAK2 in A-20 cells with mIRAK2 antibody at 1 µg/ml.
Immunocytochemistry of mIRAK2 in A-20 cells with mIRAK2 antibody at 1 µg/ml.

Immunofluorescence

Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 10 ug/ml.
Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 10 ug/ml.

Western blot

Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.

Western blot

Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.

Western blot

Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.

Western blot

Western blot of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Western blot of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Western blot

Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.

Requested From: 
Date Requested: 4/24/2018

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