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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IPCEF1 / PIP3E Antibody (aa254‑303) LS‑C82809
PIP3E antibody LS-C82809 is an unconjugated rabbit polyclonal antibody to PIP3E (IPCEF1) from human and monkey. Validated for WB.
Catalog
Size
Price
LS-C82809-50
50 µg
Unavailable

Popular IPCEF1 / PIP3E Products

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IPCEF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IPCEF1.
Species: Human
Applications: Western blot
Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA

Product Description

PIP3E antibody LS-C82809 is an unconjugated rabbit polyclonal antibody to PIP3E (IPCEF1) from human and monkey. Validated for WB.
About IPCEF1 / PIP3E
Enhances the promotion of guanine-nucleotide exchange by PSCD2 on ARF6 in a concentration-dependent manner. Q8WWN9 AB007863 NP_056368.1

IPCEF1 Antibody, PIP3-E Antibody, RP3-402L9.2 Antibody, KIAA0403 Antibody


Specifications

Target
Human IPCEF1 / PIP3E
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Predicted
Bovine, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
IPCEF1 / PIP3E antibody was raised against synthetic peptide located between aa254-303 of human IPCEF1 (Q8WWN9, NP_056368). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Bovine, Rabbit, Horse (92%); Guinea pig (85%); Mouse (78%).
Blocking Peptide
LS-E16863 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82809. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa254-303
Specificity
Human IPCEF1 / PIP3-E
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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