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Anti-INTS10 Antibody (aa52-101) LS-C187054

Catalog	Size	Price
LS-C187054-50	50 µg (1 mg/ml)	Unavailable

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Immunofluorescence analysis of A549 cells.

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Immunohistochemistry of Human cervical cancer using INTS10 Polyclonal Antibody at dilution of 1:50.

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Immunofluorescent analysis of INTS10 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Anti-INTS10 Antibody LS-C675907

Antibody:	Rabbit Polyclonal (IgG) to Human INTS10
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Immunohistochemistry of paraffin-embedded human kidney tissue at dilution 1:100

Anti-INTS10 Antibody LS-C675908

Antibody:	Rabbit Polyclonal (IgG) to Human INTS10
Reactivity:	Human
Application:	IHC, Western blot, ELISA
Format:	Unconjugated

Immunohistochemistry of paraffin-embedded human small intestine at dilution 1:100

Antibody:	Rabbit Polyclonal to Human INTS10
Reactivity:	Human, Mouse, Rat, Bovine, Rabbit
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Western blot of Human MDA-MB-435S. INTS10 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Target:	Human INTS10
Host:	Rabbit
Reactivity:	Human, Mouse, Rat, Bovine, Rabbit (tested or 100% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

INTS10 antibody was raised against synthetic peptide located between aa52-101 of human INTS10 (Q9NVR2, NP_060612). Percent identity by BLAST analysis: Human, Chimpanzee, Mouse, Rat, Bovine, Rabbit (100%).

Blocking Peptide:

LS-E25048 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C187054. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa52-101

Specificity:

Human INTS10

Packaging

Presentation:	Lyophilized from PBS
Reconstitution:	50 µl Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About INTS10

Uniprot: Q9NVR2 NCBI: NM_018142 NP_060612.2

Synonyms: INTS10 Antibody, INT10 Antibody, C8orf35 Antibody, Integrator complex subunit 10 Antibody

Description: Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

(More About INTS10)

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Western blot

Western blot of Human MDA-MB-435S. INTS10 antibody dilution 1.0 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 4/23/2018

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