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Anti-Integrin AlphaV Beta5 Antibody LS-C24771

Ordering

Wt. Vol. Conc. Price
100 µg - - $560
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Integrin AlphaV Beta5 Antibodies

Anti-Integrin AlphaV Beta5 Antibody (clone P1F6) LS-C24772
Mouse Monoclonal [clone P1F6] (IgG1) to Human Integrin AlphaV Beta5
Human, Chicken
IHC - Frozen, ICC, Immunoprecipitation, Flow Cytometry, ELISA, Functional Assay
Unconjugated
Anti-Integrin AlphaV Beta5 Antibody (clone P1F6, FITC) LS-C24775
Mouse Monoclonal [clone P1F6] (IgG1) to Human Integrin AlphaV Beta5
Human, Chicken
Flow Cytometry
FITC Conjugated
Anti-Integrin AlphaV Beta5 Antibody (clone P1F6) LS-C27262
Mouse Monoclonal [clone P1F6] (IgG1) to Human Integrin AlphaV Beta5
Human, Chicken
IHC - Frozen, Immunofluorescence, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2a) to Human Integrin AlphaV Beta5
Human
ICC, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated

Details

Human Integrin AlphaV Beta5
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Unconjugated
Purified
Unmodified

Applications

  • ICC
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA

Specificity and Use

Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.
Reactive with human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. Binds to the alphaVbeta5 complex. Has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding to alphaVbeta5. Does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.
Immunoprecipitation. Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X105 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody. Immunocytochemistry: 15 mg/ml dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody. ELISA.

Packaging

PBS. No preservative added.
For research use only.

About Integrin AlphaV Beta5


Requested From: United States
Date Requested: 12/9/2016

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