Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal (IgG3) to Mouse Integrin Alpha5 Beta1
Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Mouse Integrin Alpha5 Beta1
Mouse (tested or 100% immunogen sequence identity)
Specificity and Use
Mouse embryonal carcinoma P19 cells.
Recognizes mouse integrin VLA-5 (a5b1), a fibronectin receptor.
Suitable for use in the inhibition of cell adhesion to fibronectin, Immunoprecipitation, Flow Cytometry and Western Blot. Inhibition of Cell Adhesion to Fibronectin: 10 ul/ml Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (a4b1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete. Immunoprecipitation from lysate of 1E6 cell equivalent: 2 ul Flow cytometry: 10 ul/ml. Western Blot: non-denaturing gel.
No stabilizing proteins added. No preservative added.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C