Human Integrin Alpha V Beta 3
Human, Monkey, Bovine, Dog, Pig, Rabbit, Chicken, Avian
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- IHC - Frozen
- Immunofluorescence (5 - 10 µg/ml)
- Flow Cytometry
- (applications tested for the base form of this product only)
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Purified adhesion receptor from the human melanoma cell line M21.
Recognizes the human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species cross-reactivity: bovine, chicken, monkey, porcine, canine, rabbit and avian. Not reactive with rat tissue.
Suitable for use in Immunoprecipitation, Immunofluorescence, Flow Cytometry, Immunohistochemistry (frozen sections only), Immunocytochemistry and Blocking. Immunoprecipitation: Precipitates integrin alpha V beta3 complex. When resolved by SDS-PAGE under reducing conditions, bands are observed at ~130kD corresponding to the alpha V subunit and at ~105kD corresponding to the beta3 subunit. Flow Cytometry: 1-3 ug/0.5x1E6cells in 100 ul volume. Immunofluorescence: 5-10 ug/ml for staining of bFGF-treated 6mm cryosections of chick chorioallantoic membrane fixed with acetone. Also effective on 4% paraformaldehyde fixed, frozen tissues and cells. Not effective for immunohistochemical staining of paraffin-embedded tissue sections. Blocking: Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 ug/ml. Positive control: Human myeloma cell lines M21 and MoalphaV.
PBS, pH 7.1. No preservatives added.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.