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Anti-Insulin Antibody (clone 8E2, HRP) IHC-plus™ LS-B2407

Note: This antibody replaces LS-C24662

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Insulin Antibodies

Anti-Insulin Antibody (clone 8E2, HRP) IHC-plus™ LS-B2407
Mouse Monoclonal [clone 8E2] (IgG1,k) to Human Insulin
Human
IHC - Paraffin, ELISA
HRP Conjugated
Immunohistochemistry Image
Anti-Insulin Antibody IHC-plus™ LS-B2526
Rabbit Polyclonal (IgG) to Human Insulin
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-Insulin Antibody LS-C190934
Guinea pig Polyclonal (IgG) to Human Insulin
Human, Bovine, Pig
ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 8E2] (IgG1,k) to Human Insulin
Human
IHC - Paraffin, ELISA
HRP Conjugated

Details

Human Insulin
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [8E2]
Horseradish Peroxidase
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • ELISA

Specificity and Use

Insulin antibody was raised against purified human insulin.
Recognizes human Insulin. Species cross-reactivity: human proinsulin, bovine insulin (30%) and porcine insulin. Does not cross-react with free C-peptide.
Immunohistochemistry: LS-B2407 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2407 was determined to be 10 ug/ml. Recommended as a detection antibody for ELISA and in sandwich assay with clone 3A6 as the capture antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, pH 7.4, 0.05% Proclin 300
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles and prolonged exposure to light.
For research use only.

About Insulin

P01308 NM_000207 NP_000198.1

INS Antibody, IRDN Antibody, IDDM2 Antibody, Preproinsulin Antibody, ILPR Antibody, Insulin Antibody, MODY10 Antibody, Proinsulin Antibody

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified.

Immunohistochemistry

Immunohistochemistry
Anti-Insulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2407 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/3/2016

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