Human Insulin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

2D11.H5

Unconjugated

Protein A purified

Unmodified

This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).

Reacts specifically with insulin from human and swine sources. Cross reactivity with insulin from mouse and rat does not occur. Cross reactivity with insulin from other sources has not been determined.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:50 - 1:200)
• Immunoprecipitation
• ELISA (1:5000 - 1:25000)
• Dot Blot

Immunohistochemistry: LS-B1445 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1445 was determined to be 5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01308 NCBI: NM_000207 NP_000198.1