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Anti-Insulin Antibody (clone 2D11.H5) IHC-plus™ LS-B1445

Note: This antibody replaces LS-C18823


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular Insulin Antibodies

Anti-Insulin Antibody (clone 8E2, HRP) IHC-plus™ LS-B2407
Mouse Monoclonal [clone 8E2] (IgG1,k) to Human Insulin
IHC - Paraffin, ELISA
HRP Conjugated
Immunohistochemistry Image
Anti-Insulin Antibody IHC-plus™ LS-B2526
Rabbit Polyclonal (IgG) to Human Insulin
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Flow Cytometry
Immunohistochemistry Image
Anti-Insulin Antibody LS-C190934
Guinea pig Polyclonal (IgG) to Human Insulin
Human, Bovine, Pig

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2D11.H5] (IgG1,k) to Human Insulin
IHC - Paraffin, Western blot, ELISA


Human Insulin
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [2D11.H5]
Protein A purified


  • IHC - Paraffin (5 µg/ml)
  • Western blot

Specificity and Use

Insulin antibody was raised against fusion protein from human INS / Insulin.
Purified human insulin coupled to bovine serum albumin (BSA). Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with insulin from human pancreas using conventional hybridoma technology.
Immunohistochemistry: LS-B1445 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1445 was determined to be 5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About Insulin

P01308 NM_000207 NP_000198.1

INS Antibody, IRDN Antibody, IDDM2 Antibody, Preproinsulin Antibody, ILPR Antibody, Insulin Antibody, MODY10 Antibody, Proinsulin Antibody

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified.


Anti-Insulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1445 concentration 5 ug/ml.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Anti-Insulin Antibody - ELISA. ELISA Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1ug of conjugate. The starting concentration of antibody in the dilution series was 10 ug/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approximately 5 ng/ml antibody or a 1:200000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L(LS-C60680) and TMB substrate were used for detection.

Dot Blot

Dot Blot
Anti-Insulin Antibody - Dot Blot. Dot blotting. Mab anti-Insulin antibody (clone 2D11. H5) is shown to detect human insulin by dot blot. Each dot blot represents 1 ul of non-denatured human insulin at various dilutions starting at 1.0 ug/ml spotted on to nitrocellulose. A 1:400 dilution of Mab anti-Insulin is used for 2 hour followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] ( reacted for 45 min at room temperature and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.

Requested From: United States
Date Requested: 10/26/2016

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