(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
INSIG1 antibody was raised against a synthetic peptide representing an internal region of the human protein (between residues 1-100) according to Swiss-Prot# O15503
Immunohistochemistry: LS-B2420 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2420 was determined to be 5 ug/ml.
PBS, 0.1% Sodium Azide, 30% Glycerol
Store at 4°C or -20°C. Avoid freeze-thaw cycles.