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Anti-INSIG1 Antibody (aa1-100) IHC-plus™ LS-B2420

Note: This antibody replaces LS-C49148
Catalog Size Price
LS-B2420-50 50 µl (1.02 mg/ml) Unavailable

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Immunohistochemistry Image
Anti-INSIG1 Antibody LS-C672360
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Anti-INSIG1 Antibody (Biotin) LS-C672361
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Anti-INSIG1 Antibody (HRP) LS-C672364
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100% Guaranteed
Rabbit Polyclonal to Human INSIG1
IHC - Paraffin, Western blot (applications tested for the base form of this product only)


Human INSIG1
Human (tested or 100% immunogen sequence identity)
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Immunoaffinity purified


  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

INSIG1 antibody was raised against a synthetic peptide representing an internal region of the human protein (between residues 1-100) according to Swiss-Prot# O15503
Insig1 but not Insig2.
Immunohistochemistry: LS-B2420 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2420 was determined to be 5 ug/ml.


PBS plus 30% glycerol, 0.1% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About INSIG1

O15503 NM_005542 NP_005533.2

INSIG1 Antibody, Cl-6 Antibody, CL6 Antibody, INSIG-1 Antibody, Insulin induced gene 1 Antibody, Insulin-induced gene 1 protein Antibody, INSIG-1 membrane protein Antibody

Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78.


Anti-INSIG1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2420 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Detection of Insig 1 in HepG2 lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 3/19/2018

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