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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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INSIG1 Antibody (aa1‑100) IHC‑plus™ LS‑B2420
Note: This antibody replaces LS-C49148
INSIG1 antibody LS-B2420 is an unconjugated rabbit polyclonal antibody to human INSIG1. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2420-50
50 µl (1.02 mg/ml)
$425

Popular INSIG1 Products

Anti-INSIG1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6815 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry image of paraffin-embedded human liver cancer at a dilution of 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
INSIG1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human prostate carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry

Product Description

INSIG1 antibody LS-B2420 is an unconjugated rabbit polyclonal antibody to human INSIG1. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About INSIG1
Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. O15503 NM_005542 NP_005533.2

INSIG1 Antibody, Cl-6 Antibody, CL6 Antibody, INSIG-1 Antibody, Insulin induced gene 1 Antibody, Insulin-induced gene 1 protein Antibody, INSIG-1 membrane protein Antibody


Specifications

Target
Human INSIG1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
INSIG1 antibody was raised against a synthetic peptide representing an internal region of the human protein (between residues 1-100) according to Swiss-Prot# O15503
Epitope
aa1-100
Specificity
Insig1 but not Insig2.
Usage
Immunohistochemistry: LS-B2420 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2420 was determined to be 5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide, 30% Glycerol
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-INSIG1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2420 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-INSIG1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2420 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of Insig 1 in HepG2 lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of Insig 1 in HepG2 lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-INSIG1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2420 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-INSIG1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2420 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of Insig 1 in HepG2 lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of Insig 1 in HepG2 lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-INSIG1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2420 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-INSIG1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2420 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of Insig 1 in HepG2 lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of Insig 1 in HepG2 lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-INSIG1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2420 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-INSIG1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2420 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of Insig 1 in HepG2 lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of Insig 1 in HepG2 lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 9/24/2018

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