INSIG1 antibody LS-B2420 is an unconjugated rabbit polyclonal antibody to human INSIG1. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- IHC - Paraffin (5 µg/ml)
- Western blot (2 µg/ml)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
INSIG1 antibody was raised against a synthetic peptide representing an internal region of the human protein (between residues 1-100) according to Swiss-Prot# O15503
Insig1 but not Insig2.
Immunohistochemistry: LS-B2420 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2420 was determined to be 5 ug/ml.
PBS, 0.1% Sodium Azide, 30% Glycerol
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.