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Anti-ING5 Antibody (aa127-140) LS-C60064

Note: This antibody replaces LS-C70700

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ING5 Antibodies

Anti-ING5 Antibody (aa91-119, APC) LS-C236621
Rabbit Polyclonal (IgG) to Human ING5
Human
Western blot, ELISA
APC Conjugated
Anti-ING5 Antibody (aa91-119, AP) LS-C236623
Rabbit Polyclonal (IgG) to Human ING5
Human
Western blot, ELISA
AP Conjugated
Anti-ING5 Antibody (aa91-119, PE) LS-C236626
Rabbit Polyclonal (IgG) to Human ING5
Human
Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human ING5
Human
Western blot, ELISA
Unconjugated

Details

Human ING5
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:500 - 1:1000)
  • ELISA (1:4000 - 1:16000)

Specificity and Use

ING5 antibody was raised against synthetic peptide corresponding aa 127-140 of Human p28 ING5protein (Inhibitor of growth family, member 5). This sequence shows homology to isoforms 1 and 2 for ING5.
aa127-140
human p28 ING5 protein. The sequence is present in both isoforms 1 and 2 (see below). However, 100% homology is on record for this protein from human and chimpanzee. Cross reactivity with p28 ING5 protein from frog, mouse, rat and chicken may not occur as sequence homology varies by one or more amino acids residues in this sequence as indicated by BLAST analysis. Reactivity with p28 ING5 proteins from other sources is not known
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 28 kD or 26 kD in size corresponding to variants of ING5 by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About ING5

Q8WYH8 NM_032329 NP_115705.2

ING5 Antibody, p28ING5 Antibody, Inhibitor of growth protein 5 Antibody

Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator.

Western blot

Western blot
Anti-p28 ING5 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kD corresponding to the expected molecular weight for the recombinant protein. Approximately 10 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500. Incubation was overnight at 4° C followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Western blot
Anti-p28 ING5 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of ~45.0 kD corresponding to the expected molecular weight for the recombinant protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using chemiluminescence reagent with a 5-min exposure time. Other detection systems will yield similar results. Personal communication Jacques Cote.

Requested From: United States
Date Requested: 12/8/2016

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