Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentrations are 1 ug/ml for two hours at room temperature or 0.25 ug/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. . Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-8 (100ng/ml), a concentration of 2-3 ug/ml of this antibody is required. ELISA: To detect Human IL-8 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-8 (LS-C104790
) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-8. Western Blot: To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.