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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL8 / Interleukin 8 Antibody LS‑C104558
Note: This antibody replaces LS-C8034, LS-C54144, LS-C42975
Interleukin 8 antibody LS-C104558 is an unconjugated rabbit polyclonal antibody to human Interleukin 8 (IL8). Validated for ELISA, Neut and WB. Cited in 1 publication.
Catalog
Size
Price
LS-C104558-50
50 µg
Unavailable
LS-C104558-100
100 µg
Unavailable

Popular IL8 / Interleukin 8 Products

Antibody
Species: Human
Applications: Western blot, ELISA, Neutralization
Antibody
Species: Human
Applications: Western blot, ELISA, Neutralization
Anti-IL-8 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6427 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with IL8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

Interleukin 8 antibody LS-C104558 is an unconjugated rabbit polyclonal antibody to human Interleukin 8 (IL8). Validated for ELISA, Neut and WB. Cited in 1 publication.
About IL8 / Interleukin 8
Interleukin 8 (IL8), a member of the CXC chemokine family. This chemokine, is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. P10145 NM_000584 NP_000575.1

CXCL8 Antibody, 3-10C Antibody, AMCF-I Antibody, B-ENAP Antibody, C-X-C motif chemokine 8 Antibody, GCP-1 Antibody, IL-8 Antibody, IL8 Antibody, Interleukin-8 Antibody, K60 Antibody, LECT Antibody, GCP1 Antibody, LUCT Antibody, NAF Antibody, NAP1 Antibody, Neutrophil-activating factor Antibody, Interleukin 8 Antibody, Protein 3-10C Antibody, MDNCF Antibody, TSG-1 Antibody, Emoctakin Antibody, LYNAP Antibody, MONAP Antibody, NAP-1 Antibody, SCYB8 Antibody, T-cell chemotactic factor Antibody


Specifications

Target
Human IL8 / Interleukin 8
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization
  • (applications tested for the base form of this product only)
Immunogen
E.coli derived recombinant Human IL-8.
Specificity
Human IL-8
Usage
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentrations are 1 ug/ml for two hours at room temperature or 0.25 ug/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. . Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-8 (100ng/ml), a concentration of 2-3 ug/ml of this antibody is required. ELISA: To detect Human IL-8 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-8 (LS-C104790) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-8. Western Blot: To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-8 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (1)

1
Autocrine IL-8 promotes F-actin polymerization and mediate mesenchymal transition via ELMO1-NF-?B-Snail signaling in glioma. Zhang B, Shi L, Lu S, Sun X, Liu Y, Li H, Wang X, Zhao C, Zhang H, Wang Y. Cancer biology & therapy. 2015 16:898-911. (IHC, WB; Human) [Full Text Article] [PubMed:25870011] [PMC:PMC4623112]

Customer Reviews (0)


Images


Western blot

Western Blot (reducing) of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-8 antibody LS-C104558. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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