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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL8 / Interleukin 8 Antibody (clone 4H7C7) LS‑C203255
Interleukin 8 antibody LS-C203255 is an unconjugated mouse monoclonal antibody to human Interleukin 8 (IL8). Validated for ELISA.
Catalog
Size
Price
LS-C203255-40
40 µg (0.5 mg/ml)
Unavailable

Popular IL8 / Interleukin 8 Products

Antibody
Species: Human
Applications: Western blot, ELISA, Neutralization
Antibody
Species: Human
Applications: Western blot, ELISA, Neutralization
Western Blot (reducing) of IL-8 antibody LS-C104558.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Anti-IL-8 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6427 concentration 5 ug/ml.
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Applications: IHC, IHC - Paraffin, Western blot, ELISA
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with IL8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

Interleukin 8 antibody LS-C203255 is an unconjugated mouse monoclonal antibody to human Interleukin 8 (IL8). Validated for ELISA.
About IL8 / Interleukin 8
Interleukin 8 (IL8), a member of the CXC chemokine family. This chemokine, is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. P10145 NM_000584 NP_000575.1

CXCL8 Antibody, 3-10C Antibody, AMCF-I Antibody, B-ENAP Antibody, C-X-C motif chemokine 8 Antibody, GCP-1 Antibody, IL-8 Antibody, IL8 Antibody, Interleukin-8 Antibody, K60 Antibody, LECT Antibody, GCP1 Antibody, LUCT Antibody, NAF Antibody, NAP1 Antibody, Neutrophil-activating factor Antibody, Interleukin 8 Antibody, Protein 3-10C Antibody, MDNCF Antibody, TSG-1 Antibody, Emoctakin Antibody, LYNAP Antibody, MONAP Antibody, NAP-1 Antibody, SCYB8 Antibody, T-cell chemotactic factor Antibody


Specifications

Target
Human IL8 / Interleukin 8
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [4H7C7]
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • ELISA
Immunogen
Genetic immunization, amino acids sequence: MTSKLAVALL AAFLISAALC EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH CANTEIIVKL SDGRELCLDP KENWVQRVVE KELKRAENS
Specificity
Human IL-8 and does not cross-react with IL-1 alpha, IL-1 beta, IL-4, IL-6, and IL-18.
Usage
ELISA capture 0.5-10 ug/ml, ELISA detection 0.05-0.2 ug/ml
Presentation
Lyophilized from PBS, pH 7.4, 0.02% sodium azide.
Reconstitution
80 µl Distilled water
Storage
Store lyophilized at -20°C. The reconstituted product can be stored for short term at 4°C or long term at -20°C or below. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (4H7C7), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD
Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (4H7C7), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2.Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 1A7C8, 2D11C10 or 4H8F8) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.
Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2.Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 1A7C8, 2D11C10 or 4H8F8) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.

Western blot

Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (4H7C7), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD
Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (4H7C7), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2.Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 1A7C8, 2D11C10 or 4H8F8) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.
Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2.Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 1A7C8, 2D11C10 or 4H8F8) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.

Western blot

Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (4H7C7), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD
Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (4H7C7), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2.Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 1A7C8, 2D11C10 or 4H8F8) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.
Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2.Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 1A7C8, 2D11C10 or 4H8F8) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.

Western blot

Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (4H7C7), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD
Western blot analysis of human recombinant IL-8 fusion protein using Human IL-8 Antibody (4H7C7), mAb, Mouse The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG. Predicted Size: 36 KD Observed Size: 36 KD

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2.Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 1A7C8, 2D11C10 or 4H8F8) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.
Sandwich ELISA analysis of matched antibody pairs using Human IL-8 Antibody, mAb, Mouse 1. ELISA plate is coated with Human IL-8 Antibody, mAb, Mouse ). 2.Human recombinant IL-8 protein at appropriate dilution is added into appropriate reaction wells. 3. After a period of incubation, HRP conjugated Human IL-8 Antibody, mAb, Mouse (Clone. 1A7C8, 2D11C10 or 4H8F8) is added followed by proper period of incubation. 4. TMB substrate is added and developed at room temperature. 5.Stop the reaction with 1.0 N HCl and read the plate at 450nm.


Requested From: 
Date Requested: 6/23/2018

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