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Catalog Number Size Price
LS-C407834-10 10 µg $318 
LS-C407834-100 100 µg $470 
IL8 / Interleukin 8 Antibody - IHC analysis of IL-8 using anti-IL-8 antibody. IL-8 was detected in paraffin-embedded section of human appendicitis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IL-8 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IL8 / Interleukin 8 Antibody - IL8 antibody Western blot. All lanes: Anti IL8 at 0.5 ug/ml. WB: HELA Whole Cell Lysate at 40 ug. Predicted band size: 11 kD. Observed band size: 34 kD.
IL8 / Interleukin 8 Antibody - IHC analysis of IL-8 using anti-IL-8 antibody. IL-8 was detected in paraffin-embedded section of human appendicitis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IL-8 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IL8 / Interleukin 8 Antibody - IL8 antibody Western blot. All lanes: Anti IL8 at 0.5 ug/ml. WB: HELA Whole Cell Lysate at 40 ug. Predicted band size: 11 kD. Observed band size: 34 kD.
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Polyclonal Rabbit anti‑Human IL8 / Interleukin 8 Antibody (aa23‑99, WB) LS‑C407834

Polyclonal Rabbit anti‑Human IL8 / Interleukin 8 Antibody (aa23‑99, WB) LS‑C407834

Antibody:
IL8 / Interleukin 8 Rabbit anti-Human Polyclonal (aa23-99) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407834-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL8 / Interleukin 8 Rabbit anti-Human Polyclonal (aa23-99) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Interleukin 8 antibody LS-C407834 is an unconjugated rabbit polyclonal antibody to human Interleukin 8 (IL8) (aa23-99). Validated for ELISA and WB.
Target
Human IL8 / Interleukin 8
Synonyms
CXCL8 | 3-10C | AMCF-I | B-ENAP | C-X-C motif chemokine 8 | GCP-1 | IL-8 | IL8 | Interleukin-8 | K60 | LECT | GCP1 | LUCT | NAF | NAP1 | Neutrophil-activating factor | Interleukin 8 | Protein 3-10C | MDNCF | TSG-1 | Emoctakin | LYNAP | MONAP | NAP-1 | SCYB8 | T-cell chemotactic factor
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human IL-8 recombinant protein (Position: A23-S99).
Epitope
aa23-99
Applications
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL8 / Interleukin 8
P10145 NM_000584 NP_000575.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024