LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-IL7R / CD127 Antibody (phospho-Tyr449) IHC-plus™ LS-B2831

Note: This antibody replaces LS-C60126

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL7R / CD127 Antibodies

Anti-IL7R / CD127 Antibody (C-Terminus) IHC-plus™ LS-B2830
Rabbit Polyclonal to Mouse IL7R / CD127
Mouse, Human, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL7R / CD127 Antibody (phospho-Tyr449) IHC-plus™ LS-B2831
Rabbit Polyclonal to Mouse IL7R / CD127
Mouse, Human, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL7R / CD127 Antibody (clone ANC8F2, RPE) LS-C134720
Mouse Monoclonal [clone ANC8F2] (IgG1,k) to Human IL7R / CD127
Human
Flow Cytometry
RPE Conjugated
Flow Cytometry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse IL7R / CD127
Mouse, Human, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Mouse IL7R / CD127
Rabbit
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000 - 1:10000)
  • ELISA (1:100000)

Specificity and Use

IL7R / CD127 antibody was raised against synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
pTyr449
A BLAST analysis was used to suggest cross-reactivity with IL-7R from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7R from other sources has not been determined.
Immunohistochemistry: LS-B2831 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2831 was determined to be 5 ug/ml. Western Blot: Expect a band approximately 55 kD in size corresponding to phosphorylated IL-7 in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About IL7R / CD127

P16871 NM_002185 NP_002176.2

IL7R Antibody, CD127 Antibody, CD127 antigen Antibody, IL-7RA Antibody, IL-7 receptor subunit alpha Antibody, IL7RA Antibody, ILRA Antibody, Interleukin 7 receptor Antibody, CDW127 Antibody, IL-7R subunit alpha Antibody, IL-7R-alpha Antibody

IL7R / CD127 is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation.

Immunohistochemistry

Immunohistochemistry
Anti-IL-7 Receptor antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2831 concentration 5 ug/ml.

Western blot

Western blot
Anti-IL-7 Receptor alpha chain pY449 - Western Blot. Anti-IL-7 Receptor alpha chain pY449 in immunoprecipitation and Western blot. Lysates were prepared from thymocyte D1 cells treated with or without IL7 (50 ng/ml), and immunoprecipitated with anti-Phosphotyrosine conjugated to Protein G agarose. After washing and boiling, the supernatant was separated by SDS-PAGE and transferred to nitrocellulose. Western Blot analysis was performed using anti-IL7 receptor pY449, diluted 1:10000 in BSA, incubated overnight at 4°C. The proteins were visualized using an HRP-conjugated goat anti-rabbit antibody and ECL detection.

Requested From: United States
Date Requested: 12/9/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number