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Anti-IL7 Antibody LS-C154092


Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular IL7 Antibodies

Anti-IL7 Antibody LS-C8012
Rabbit Polyclonal (IgG) to Mouse IL7
Western blot, ELISA, Functional Assay, Neutralization

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human IL7
Western blot, ELISA


Human IL7
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Ion exchange chromatography


  • Western blot (1:1000)

Specificity and Use

IL7 antibody was raised against this purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-7 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 17.4 kD in size corresponding to native human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL7

P13232 NM_000880 NP_000871.1

IL7 Antibody, IL-7 Antibody, Interleukin-7 Antibody, Interleukin 7 Antibody

IL7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes.

Western blot

Western blot
Anti-IL-7 Antibody - Western Blot. anti-Human IL-7 antibody shows detection of a band ~17 kD in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

Requested From: United States
Date Requested: 10/21/2016

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