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Anti-IL7 Antibody (Biotin) LS-C104559

Note: This antibody replaces LS-C8014, LS-C54141, LS-C42705

Ordering

Wt. Vol. Conc. Price
25 µg - - $285
50 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL7 Antibodies

Anti-IL7 Antibody LS-C8012
Rabbit Polyclonal (IgG) to Mouse IL7
Mouse
Western blot, ELISA, Functional Assay, Neutralization
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human IL7
Human
Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Biotin Conjugated

Details

Human IL7
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin. Also available Unconjugated.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
  • Neutralization
  • (applications tested for the base form of this product only)

Specificity and Use

IL7 antibody was raised against e.coli derived recombinant Human IL-7.
Human IL-7
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human IL-7 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-7. To detect Human IL-7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-7 (LS-C104554) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-7. Western Blot: To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant hIL-7 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL7

P13232 NM_000880 NP_000871.1

IL7 Antibody, IL-7 Antibody, Interleukin-7 Antibody, Interleukin 7 Antibody

IL7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes.

Western blot

Western blot
Western Blot (non-reducing) of Interleukin 7 / IL-7 antibody LS-C104554. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of Interleukin 7 / IL-7 antibody LS-C104554. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of Interleukin 7 / IL-7 antibody LS-C104559 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Direct ELISA of Interleukin 7 / IL-7 antibody LS-C104559 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of Interleukin 7 / IL-7 antibody LS-C104554. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/2/2016

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