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Anti-IL7 Antibody (Biotin) LS-C154121

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL7 Antibodies

Anti-IL7 Antibody LS-C8012
Rabbit Polyclonal (IgG) to Mouse IL7
Mouse
Western blot, ELISA, Functional Assay, Neutralization
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human IL7
Human
IHC, Western blot, ELISA
Biotin Conjugated

Details

Human IL7
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Biotin
Ion exchange chromatography
Unmodified

Applications

  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA

Specificity and Use

IL7 antibody was raised against this purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-7 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 17.4 kD in size corresponding to human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% sodium azide
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL7

P13232 NM_000880 NP_000871.1

IL7 Antibody, IL-7 Antibody, Interleukin-7 Antibody, Interleukin 7 Antibody

IL7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes.

Western blot

Western blot
Biotin Rabbit Anti-Human IL-7 Antibody - Western Blot. Western Blot showing detection of Human IL-7. 50 ng of Human IL-7 (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20°C, Anti-Human IL-7 (RABBIT) Antibody Biotin Conjugated LS-C154121 secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 17 kD molecular weight position expected for Human IL-7. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/8/2016

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