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Antibodies > IL6 / Interleukin 6 > LS-C355485
 
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Catalog Number Size Price
LS-C355485-1 1 mg $493 
IL6 / Interleukin 6 Antibody - Western blot analysis of IL-6 was performed by loading 20µg of LPS-treated THP-1 whole cell lysates in non-reducing sample buffer and 8ul PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter and blocked with 5% Milk/TBST for at least 1 hour at room temperature. IL-6 was detected using a IL-6 rabbit polyclonal antibody at a dilution of 1:1000 in blocking buffer overnight at 4°C on a rocking platform, followed by a HRP conjugated secondary antibody at a dilution of 1:5000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura Extended Duration Substrate and the myECL Imager.
IL6 / Interleukin 6 Antibody - Flow cytometry analysis of IL-6 on LPS-treated THP-1 cells. Cells were fixed with 4% formaldehyde for 30 min on ince and permeabilized with IC permeabilization buffer. After incubation with blocking buffer for 30 min on ice, cells were then stained with IFN-gamma rabbit polyclonal antibody or rabbit IgG control at 1:100 dilution with IC permeabilization buffer for 30 min on ice. After washing with ice-cold IC permeabilization buffer for 3 times, the cells were stained with DyLight 488 goat anti-mouse secondary antibody for 30 min on ice. A representative 10,000 cells were acquired for each sample.
IL6 / Interleukin 6 Antibody - Flow cytometry analysis of IL-6 on LPS-treated THP-1 cells. Cells were fixed with 4% formaldehyde for 30 min on ince and permeabilized with IC permeabilization buffer. After incubation with blocking buffer for 30 min on ice, cells were then stained with IFN-gamma rabbit polyclonal antibody at indicated dilution with IC permeabilization buffer for 30 min on ice. After washing with ice-cold IC permeabilization buffer for 3 times, the cells were stained with DyLight 488 goat anti-mouse secondary antibody for 30 min on ice. A representative 10,000 cells were acquired for each sample.
IL6 / Interleukin 6 Antibody - Direct ELISA analysis of LPS-treated IL-6 was performed by coating wells of a 96-well plate with 100µl per well of IL-6 diluted in carbonate/bicarbonate buffer at a concentration of 2 µg/ml overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer, and incubated with 100ul per well of a rabbit anti-IL-6 oligoclonal antibody at a serially dilution of 1:50, 1:100, 1:250, 1:500, 1:1000, 1:2000 and 1:5000 for 90 minutes at 37C. The plate was washed, then incubated with 100µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:5000 for 90 minutes at 37C. Detection was performed using 1-Step Ultra TMB substrate for 5-10 minutes at room temperature in the dark. The reaction was stopped with Stop solution, and absorbances were read on a spectrophotometer at 450-550nm.
IL6 / Interleukin 6 Antibody - Western blot analysis of IL-6 was performed by loading 20µg of LPS-treated THP-1 whole cell lysates in non-reducing sample buffer and 8ul PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter and blocked with 5% Milk/TBST for at least 1 hour at room temperature. IL-6 was detected using a IL-6 rabbit polyclonal antibody at a dilution of 1:1000 in blocking buffer overnight at 4°C on a rocking platform, followed by a HRP conjugated secondary antibody at a dilution of 1:5000 for at least 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura Extended Duration Substrate and the myECL Imager.
IL6 / Interleukin 6 Antibody - Flow cytometry analysis of IL-6 on LPS-treated THP-1 cells. Cells were fixed with 4% formaldehyde for 30 min on ince and permeabilized with IC permeabilization buffer. After incubation with blocking buffer for 30 min on ice, cells were then stained with IFN-gamma rabbit polyclonal antibody or rabbit IgG control at 1:100 dilution with IC permeabilization buffer for 30 min on ice. After washing with ice-cold IC permeabilization buffer for 3 times, the cells were stained with DyLight 488 goat anti-mouse secondary antibody for 30 min on ice. A representative 10,000 cells were acquired for each sample.
IL6 / Interleukin 6 Antibody - Flow cytometry analysis of IL-6 on LPS-treated THP-1 cells. Cells were fixed with 4% formaldehyde for 30 min on ince and permeabilized with IC permeabilization buffer. After incubation with blocking buffer for 30 min on ice, cells were then stained with IFN-gamma rabbit polyclonal antibody at indicated dilution with IC permeabilization buffer for 30 min on ice. After washing with ice-cold IC permeabilization buffer for 3 times, the cells were stained with DyLight 488 goat anti-mouse secondary antibody for 30 min on ice. A representative 10,000 cells were acquired for each sample.
IL6 / Interleukin 6 Antibody - Direct ELISA analysis of LPS-treated IL-6 was performed by coating wells of a 96-well plate with 100µl per well of IL-6 diluted in carbonate/bicarbonate buffer at a concentration of 2 µg/ml overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer, and incubated with 100ul per well of a rabbit anti-IL-6 oligoclonal antibody at a serially dilution of 1:50, 1:100, 1:250, 1:500, 1:1000, 1:2000 and 1:5000 for 90 minutes at 37C. The plate was washed, then incubated with 100µl per well of an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:5000 for 90 minutes at 37C. Detection was performed using 1-Step Ultra TMB substrate for 5-10 minutes at room temperature in the dark. The reaction was stopped with Stop solution, and absorbances were read on a spectrophotometer at 450-550nm.
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Polyclonal Rabbit anti‑Human IL6 / Interleukin 6 Antibody (IHC, WB) LS‑C355485

Polyclonal Rabbit anti‑Human IL6 / Interleukin 6 Antibody (IHC, WB) LS‑C355485

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Antibody:
IL6 / Interleukin 6 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$493
LS-C355485-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IL6 / Interleukin 6 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, Flo, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Interleukin 6 antibody LS-C355485 is an unconjugated rabbit polyclonal antibody to human Interleukin 6 (IL6). Validated for ELISA, Flow, IHC, Neut and WB.
Target
Human IL6 / Interleukin 6
Synonyms
IL6 | B-cell stimulatory factor 2 | BSF-2 | BSF2 | CTL differentiation factor | Hybridoma growth factor | IFN-beta-2 | Interleukin-6 | Interleukin BSF-2 | IFNB2 | B-cell differentiation factor | CDF | HSF | IL-6
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human IL-6.
Specificity
The antibody targets IL-6 in ELISA, IB, IHC(P), and Neu applications and shows reactivity with Human samples. It detects IL-6 which has a predicted molecular weight of approximately 22 kD.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • Flow Cytometry
  • ELISA
  • Neutralization
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, no preservative.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL6 / Interleukin 6
P05231 NM_000600 NP_000591.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
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