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Anti-IL6 / Interleukin 6 Antibody (HRP) LS-C154100

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL6 / Interleukin 6 Antibodies

Anti-IL6 / Interleukin 6 Antibody (clone 6D9A1,6C12B1) IHC-plus™ LS-B1582
Mouse Monoclonal [clone 6D9A1,6C12B1] (IgG1) to Human IL6 / Interleukin 6
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL6 / Interleukin 6 Antibody IHC-plus™ LS-B7482
Rabbit Polyclonal (IgG) to Human IL6 / Interleukin 6
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-IL6 / Interleukin 6 Antibody (clone 10E5) IHC-plus™ LS-B5763
Mouse Monoclonal [clone 10E5] (IgG2b) to Rat IL6 / Interleukin 6
Rat
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human IL6 / Interleukin 6
Human
IHC, Western blot, ELISA
HRP Conjugated

Details

Human IL6 / Interleukin 6
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Horseradish Peroxidase
Purified, heated to 56°C for 30 minutes
Unmodified

Applications

  • IHC (1:500 - 1:2500)
  • Western blot (1:1000 - 1:5000)
  • ELISA

Specificity and Use

IL6 / Interleukin 6 antibody was raised against this purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E. coli.
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 23.7 kD in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL6 / Interleukin 6

P05231 NM_000600 NP_000591.1

IL6 Antibody, B-cell stimulatory factor 2 Antibody, BSF-2 Antibody, BSF2 Antibody, CTL differentiation factor Antibody, Hybridoma growth factor Antibody, IFN-beta-2 Antibody, Interleukin-6 Antibody, Interferon beta-2 Antibody, Interleukin BSF-2 Antibody, IFNB2 Antibody, B-cell differentiation factor Antibody, CDF Antibody, HSF Antibody, IL-6 Antibody

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine.

Western blot

Western blot
Western Blot of Peroxidase conjugated Rabbit anti-Human IL-6 antibody. Lane 1: Human IL-6. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase Human IL-6 secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 20 kDa for Human IL-6. Other band(s): none. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/8/2016

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