Human IL6 / Interleukin 6

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with mature length recombinant human IL-6 produced in E.coli.

This product has minimal reactivity with mouse IL-6.

• IHC (1:200 - 1:1000)
• Western blot (1:300 - 1:1000)
• ELISA (1:10000)

This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 23.7 kD in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl

Reconstitute in 100 µL of deionized water.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05231 NCBI: NM_000600 NP_000591.1