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Anti-IL6 / Interleukin 6 Antibody (clone 33A12.G9) LS-C153821

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL6 / Interleukin 6 Antibodies

Anti-IL6 / Interleukin 6 Antibody (clone 6D9A1,6C12B1) IHC-plus™ LS-B1582
Mouse Monoclonal [clone 6D9A1,6C12B1] (IgG1) to Human IL6 / Interleukin 6
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL6 / Interleukin 6 Antibody (clone 10E5) IHC-plus™ LS-B5763
Mouse Monoclonal [clone 10E5] (IgG2b) to Rat IL6 / Interleukin 6
Rat
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-IL6 / Interleukin 6 Antibody IHC-plus™ LS-B7482
Rabbit Polyclonal (IgG) to Human IL6 / Interleukin 6
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 33A12.G9] (IgG) to Human IL6 / Interleukin 6
Human
Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human IL6 / Interleukin 6
Mouse
Human (tested or 100% immunogen sequence identity)
IgG Monoclonal [33A12.G9]
Unconjugated
Purified
Unmodified

Applications

  • Western blot (1:1000)
  • Flow Cytometry
  • ELISA

Specificity and Use

IL6 / Interleukin 6 antibody was raised against this Protein A purified monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E. coli followed by hybridoma development.
This purified antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (i. e. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.
This purified antibody has been tested for use in ELISA, Flow Cytometry, and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 23.7 kD in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL6 / Interleukin 6

P05231 NM_000600 NP_000591.1

IL6 Antibody, B-cell stimulatory factor 2 Antibody, BSF-2 Antibody, BSF2 Antibody, CTL differentiation factor Antibody, Hybridoma growth factor Antibody, IFN-beta-2 Antibody, Interleukin-6 Antibody, Interferon beta-2 Antibody, Interleukin BSF-2 Antibody, IFNB2 Antibody, B-cell differentiation factor Antibody, CDF Antibody, HSF Antibody, IL-6 Antibody

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine.

Western blot

Western blot
Western Blot showing detection of Human IL-6. 100 ng of Human IL-6 was run on a 4-20% gel and transferred to 0.45 m nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20C, Anti-Human IL-6 (MOUSE) Antibody (p/n LS-C153821) was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:40000 for 30 min at 20C and imaged using the Bio-Rad VersaDoc 4000 MP. Band indicates correct 17 kDa molecular weight position expected for Human IL-6.

Flow Cytometry

Flow Cytometry
Flow Cytometry of Human anti-IL-6 antibody LS-C153821 Cells: human PBMC Stimulation: Figure A: un-stimulated, Figure B: 1 g/mL LPS in a protein transport inhibitor for 5 hours Staining: (surface) x-axis: anti-CD14, (intracellular) y-axis: anti-IL-6

Requested From: United States
Date Requested: 12/2/2016

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