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Anti-IL6 / Interleukin 6 Antibody (Biotin) LS-C104546

Note: This antibody replaces LS-C7935, LS-C54131, LS-C42363

Ordering

Wt. Vol. Conc. Price
25 µg - - Unavailable
50 µg - - Unavailable

Most Popular IL6 / Interleukin 6 Antibodies

Anti-IL6 / Interleukin 6 Antibody (clone 6D9A1,6C12B1) IHC-plus™ LS-B1582
Mouse Monoclonal [clone 6D9A1,6C12B1] (IgG1) to Human IL6 / Interleukin 6
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL6 / Interleukin 6 Antibody (Internal) IHC-plus™ LS-A9691
Rabbit Polyclonal to Human IL6 / Interleukin 6
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-IL6 / Interleukin 6 Antibody LS-C70904
Rabbit Polyclonal (IgG) to Human IL6 / Interleukin 6
Human, Mouse, Rat
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA, Neutralization, Radioimmunoassay
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human IL6 / Interleukin 6
Human
Western blot, ELISA
Biotin Conjugated

Details

Human IL6 / Interleukin 6
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)

Specificity and Use

IL6 / Interleukin 6 antibody was raised against e.coli derived recombinant Human IL-6.
Human IL-6
ELISA: To detect Human IL-6 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. To detect Human IL-6 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-6 (LS-B7482) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-6. Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-6 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL6 / Interleukin 6

P05231 NM_000600 NP_000591.1

IL6 Antibody, B-cell stimulatory factor 2 Antibody, BSF-2 Antibody, BSF2 Antibody, CTL differentiation factor Antibody, Hybridoma growth factor Antibody, IFN-beta-2 Antibody, Interleukin-6 Antibody, Interferon beta-2 Antibody, Interleukin BSF-2 Antibody, IFNB2 Antibody, B-cell differentiation factor Antibody, CDF Antibody, HSF Antibody, IL-6 Antibody

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL-6 antibody LS-C104546 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Direct ELISA of IL-6 antibody LS-C104546 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 3/30/2017

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