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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL6 / Interleukin 6 Antibody (aa27‑55) LS‑C165212
Interleukin 6 antibody LS-C165212 is an unconjugated rabbit polyclonal antibody to human Interleukin 6 (IL6). Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C165212-400
400 µl
Unavailable

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Product Description

Interleukin 6 antibody LS-C165212 is an unconjugated rabbit polyclonal antibody to human Interleukin 6 (IL6). Validated for Flow, IHC and WB.
About IL6 / Interleukin 6
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. P05231 NM_000600 NP_000591.1

IL6 Antibody, B-cell stimulatory factor 2 Antibody, BSF-2 Antibody, BSF2 Antibody, CTL differentiation factor Antibody, Hybridoma growth factor Antibody, IFN-beta-2 Antibody, Interleukin-6 Antibody, Interferon beta-2 Antibody, Interleukin BSF-2 Antibody, IFNB2 Antibody, B-cell differentiation factor Antibody, CDF Antibody, HSF Antibody, IL-6 Antibody


Specifications

Target
Human IL6 / Interleukin 6
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E1702 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C165212. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa27-55
Specificity
This IL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the Central region of human IL6.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IL6 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IL6 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IL6 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The IL6 antibody detected the IL6 protein (arrow).
IL6 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The IL6 antibody detected the IL6 protein (arrow).

Flow Cytometry

IL6 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL6 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

IL6 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IL6 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IL6 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The IL6 antibody detected the IL6 protein (arrow).
IL6 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The IL6 antibody detected the IL6 protein (arrow).

Flow Cytometry

IL6 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL6 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

IL6 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IL6 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IL6 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The IL6 antibody detected the IL6 protein (arrow).
IL6 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The IL6 antibody detected the IL6 protein (arrow).

Flow Cytometry

IL6 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL6 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

IL6 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
IL6 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

IL6 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The IL6 antibody detected the IL6 protein (arrow).
IL6 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The IL6 antibody detected the IL6 protein (arrow).

Flow Cytometry

IL6 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL6 Antibody flow cytometry of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/20/2018

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