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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL5 Antibody (Biotin) LS‑C104567
Note: This antibody replaces LS-C7901, LS-C54129, LS-C42359
IL5 antibody LS-C104567 is a biotin-conjugated rabbit polyclonal antibody to human IL5. Validated for ELISA and WB.
Catalog
Size
Price
LS-C104567-25
25 µg
Unavailable
LS-C104567-50
50 µg
Unavailable

Popular IL5 Products

Antibody
Species: Human
Applications: Western blot, ELISA, Functional Assay
Antibody
Species: Human
Applications: Western blot, ELISA
Western Blot (reducing) of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Western blot, ELISA, Neutralization
Antibody
Species: Monkey
Applications: Western blot
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

IL5 antibody LS-C104567 is a biotin-conjugated rabbit polyclonal antibody to human IL5. Validated for ELISA and WB.
About IL5
IL5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. P05113 NM_000879 NP_000870.1

IL5 Antibody, EDF Antibody, Interleukin-5 Antibody, IL-5 Antibody, T-cell replacing factor Antibody, TRF Antibody


Specifications

Target
Human IL5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
IL5 antibody was raised against e.coli derived recombinant Human IL-5.
Specificity
Human IL-5
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human IL-5 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. To detect Human IL-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-5 (LS-C104699) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. Western Blot: To detect Human IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile PBS containing 0.1% BSA
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot (non-reducing) of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Direct ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Direct ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Direct ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Direct ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Direct ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Direct ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Direct ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Direct ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-5 antibody LS-C104699.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/17/2018

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