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Anti-IL5 Antibody (Biotin) LS-C104567

Note: This antibody replaces LS-C7901, LS-C54129, LS-C42359

Ordering

Wt. Vol. Conc. Price
25 µg - - $285
50 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL5 Antibodies

Anti-IL5 Antibody LS-C104699
Rabbit Polyclonal to Human IL5
Human
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human IL5
Human
IHC, Western blot, ELISA (applications tested for the base form of this product only)
Biotin Conjugated

Details

Human IL5
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin. Also available Unconjugated.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

IL5 antibody was raised against e.coli derived recombinant Human IL-5.
Human IL-5
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Human IL-5 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. To detect Human IL-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-5 (LS-C104699) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. Western Blot: To detect Human IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL5

P05113 NM_000879 NP_000870.1

IL5 Antibody, EDF Antibody, Interleukin-5 Antibody, IL-5 Antibody, T-cell replacing factor Antibody, TRF Antibody

IL5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases.

Immunohistochemistry

Immunohistochemistry
IHC of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Direct ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL-5 antibody LS-C104567 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL-5 antibody LS-C104699. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/8/2016

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