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Anti-IL4 Antibody (clone 8D4-8) LS-C107125

Ordering

Wt. Vol. Conc. Price
50 µg - - $215
500 µg - - $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL4 Antibodies

Anti-IL4 Antibody (clone 860A 4B3) LS-C7845
Mouse Monoclonal [clone 860A 4B3] (IgG1,k) to Human IL4
Human
Immunoprecipitation, Flow Cytometry, ELISA, Sandwich ELISA
Unconjugated
Anti-IL4 Antibody IHC-plus™ LS-B7474
Rabbit Polyclonal (IgG) to Human IL4
Human
IHC - Paraffin, Western blot, ELISA, Neutralization, Sandwich ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 8D4-8] (IgG1,k) to Human IL4
Human
ELISA, ELISPOT
Unconjugated

Details

Human IL4
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [8D4-8]
Unconjugated
Affinity purified
Unmodified

Applications

  • ELISA
  • ELISPOT

Specificity and Use

IL4 antibody was raised against human IL4
A suitable range of concentrations of this antibody for ELISA capture is 1-4 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/ml - 4 pg/ml should be included in each ELISA plate. The fluorochrome-conjugated 8D4-8 antibody is offered in 2 formats:- ug size: has been tested by intracellular staining for flow cytometric analysis. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining for flow cytometric analysis. This can be used at 20 ul per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Packaging

PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
Store at 4°C, avoid repeated freeze thaw cycles.
For research use only.

About IL4

P05112 NM_000589 NP_000580.1

IL4 Antibody, B cell growth factor 1 Antibody, B-cell stimulatory factor 1 Antibody, B_cell stimulatory factor 1 Antibody, BCGF1 Antibody, BSF-1 Antibody, Binetrakin Antibody, BCGF-1 Antibody, Interleukin 4 Antibody, Interleukin-4 Antibody, Pitrakinra Antibody, BSF1 Antibody, IL-4 Antibody

IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine.

Flow Cytometry

Flow Cytometry
Human peripheral blood mononuclear cells were stimulated with PMA and ionomycin in the presence of Brefeldin A for 5 hours. The cells were surface stained with FITC anti-human CD3 (UCHT1) and intracellularly stained with PE anti-human IL-4 (8D4-8).
 

Requested From: United States
Date Requested: 12/2/2016

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