Human IL4

Rat

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

Biotin

Purified

Unmodified

CHO-expressed recombinant human IL-4.

Recognizes human Interleukin-4 (IL-4). Has been reported to cross-react with IL-4 from Rhesus monkeys. Has also been reported to be useful for epitope mapping of human IL-4.

• Immunofluorescence
• Western blot
• Flow Cytometry
• ELISA

ELISA Detection: Monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL-4 protein levels.1,3 Biotinylated antibody should be paired with a suitable purified antibody as the capture antibody, with purified recombinant human IL-4 as the standard. Immunofluorescence/. Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant human IL-4, unlabeled antibody, or Biotinylated Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-4 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

PBS, Sodium Azide

Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05112 NCBI: NM_000589 NP_000580.1