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Anti-IL4 Antibody (Biotin) LS-C104301

Note: This antibody replaces LS-C7835, LS-C54123, LS-C42974

Ordering

Wt. Vol. Conc. Price
25 µg - - $285
50 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL4 Antibodies

Anti-IL4 Antibody (clone 860A 4B3) LS-C7845
Mouse Monoclonal [clone 860A 4B3] (IgG1,k) to Human IL4
Human
Immunoprecipitation, Flow Cytometry, ELISA, Sandwich ELISA
Unconjugated
Anti-IL4 Antibody IHC-plus™ LS-B7474
Rabbit Polyclonal (IgG) to Human IL4
Human
IHC - Paraffin, Western blot, ELISA, Neutralization, Sandwich ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human IL4
Human
Western blot, ELISA
Biotin Conjugated

Details

Human IL4
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)

Specificity and Use

IL4 antibody was raised against e.coli derived recombinant Human IL-4.
Human IL-4
ELISA: To detect hIL-4 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-4. To detect hIL-4 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-4 (LS-B7474) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-4. Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL4

P05112 NM_000589 NP_000580.1

IL4 Antibody, B cell growth factor 1 Antibody, B-cell stimulatory factor 1 Antibody, B_cell stimulatory factor 1 Antibody, BCGF1 Antibody, BSF-1 Antibody, Binetrakin Antibody, BCGF-1 Antibody, Interleukin 4 Antibody, Interleukin-4 Antibody, Pitrakinra Antibody, BSF1 Antibody, IL-4 Antibody

IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL-4 antibody LS-C104301 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Direct ELISA of IL-4 antibody LS-C104301 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/4/2016

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