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Anti-IL31 Antibody (Internal) IHC-plus™ LS-B575

Note: This antibody replaces LS-C19574
Catalog Size Price
LS-B575-50 50 µg (1 mg/ml) Unavailable

Related Products

35 IL31 Antibodies

Most Popular IL31 Antibodies

Anti-IL31 Antibody (Internal) IHC-plus™ LS-B575
Rabbit Polyclonal to Human IL31
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IL31 Antibody IHC-plus™ LS-B7477
Rabbit Polyclonal (IgG) to Human IL31
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL31 Antibody (aa13-40) LS-C165207
Rabbit Polyclonal to Human IL31
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-IL31 Antibody (aa19-49, APC) LS-C236588
Rabbit Polyclonal (IgG) to Human IL31
Human
IHC, Western blot, Flow Cytometry
APC Conjugated
Anti-IL31 Antibody (aa19-49, Azide-free, HRP) LS-C249271
Rabbit Polyclonal (IgG) to Human IL31
Human
IHC, Western blot, Flow Cytometry, ELISA
HRP Conjugated, Azide-free

100% Guaranteed
Rabbit Polyclonal to Human IL31
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human IL31
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot

Specificity and Use

IL31 antibody was raised against synthetic peptide from human IL31 / IL-31.
LS-E30214 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B575. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Internal
18 amino acid peptide from near the center of human IL-31
Immunohistochemistry: LS-B575 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B575 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About IL31

Q6EBC2 NM_001014336 NP_001014358.1

IL31 Antibody, IL-31 Antibody, Interleukin 31 Antibody, Interleukin-31 Antibody

IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma (Dillon et al., 2004 [PubMed 15184896]).

Immunohistochemistry

Immunohistochemistry
Anti-IL-31 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B575 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of IL-31 in Human Skeletal Muscle cells with IL-31 antibody at 20 ug/ml.

Western blot

Western blot
Western blot of IL-31 in human skeletal muscle tissue lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 ug/ml.

Requested From: 
Date Requested: 10/19/2017

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