Target
Human IL3
Synonyms
IL3 | Hematopoietic growth factor | IL-3 | Mast-cell growth factor | MULTI-CSF | P-cell-stimulating factor | Interleukin-3 | Mast cell growth factor | MCGF | P-cell stimulating factor
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Immunogen
Yeast-expressed recombinant human IL-3.
Specificity
Recognizes human Interleukin-3 (IL-3).
Usage
ELISA Capture: Purified monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human IL-3 protein levels.1-3 The purified antibody should be paired with biotinylated antibody as the detection antibody, with purified recombinant human IL-3 as the standard. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-3 under reducing conditions.
Presentation
100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee