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Anti-IL2RA / CD25 Antibody IHC-plus™ LS-B7396

Note: This antibody replaces LS-C104460

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2a,k) to Human IL2RA / CD25
Human
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated

Details

Human IL2RA / CD25
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal
Protein G purified

Applications

IHC - Paraffin (10 µg/ml), IHC - Frozen (5 µg/ml), Western blot (0.2 - 0.4 µg/ml), ELISA

Specificity and Use

IL2RA / CD25 antibody was raised against insect cell derived recombinant Human sIL-2Ralpha.
Human IL-2Ralpha
Immunohistochemistry: LS-B7396 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 10 ug/ml is recommended. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 100 pg/ml of recombinant Human sIL-2Ralpha when used in conjunction with compatible secondary reagents. Western Blot: To detect Human sIL-2Ralpha by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sIL-2Ralpha is 2-4 ng/lane, under non-reducing conditions.

Packaging

Lyophilized from PBS
Reconstitute in sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL2RA / CD25

P01589 NM_000417 NP_000408.1

IL2RA Antibody, CD25 antigen Antibody, CD25 Antibody, IDDM10 Antibody, IL-2 receptor subunit alpha Antibody, IL2R Antibody, Interleukin 2 receptor, alpha Antibody, IL-2-RA Antibody, IL2-RA Antibody, TCGFR Antibody, IL-2R subunit alpha Antibody, p55 Antibody, TAC antigen Antibody


Immunohistochemistry

Immunohistochemistry
Anti-CD25 antibody IHC of human plasma cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7396 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 9/4/2015

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