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| Catalog Number | Size | Price |
|---|---|---|
| LS-C45048-0.1 | 0.1 mg (1 mg/ml) | $395 |
Monoclonal Mouse anti‑Rat IL2RA / CD25 Antibody (clone OX‑39, IHC) LS‑C45048
Monoclonal Mouse anti‑Rat IL2RA / CD25 Antibody (clone OX‑39, IHC) LS‑C45048
Note: This antibody replaces LS-C45050, LS-C122007, LS-C106237
Antibody:
IL2RA / CD25 Mouse anti-Rat Monoclonal (OX-39) Antibody
Application:
IHC, IHC-P, IHC-Fr, IP, Flo, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
IL2RA / CD25 Mouse anti-Rat Monoclonal (OX-39) Antibody
Application:
IHC, IHC-P, IHC-Fr, IP, Flo, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Specifications
Description
CD25 antibody LS-C45048 is an unconjugated mouse monoclonal antibody to rat CD25 (IL2RA). Validated for ELISA, Flow, IHC and IP.
Target
Rat IL2RA / CD25
Synonyms
IL2RA | CD25 antigen | CD25 | IDDM10 | IL-2 receptor subunit alpha | IL2R | Interleukin 2 receptor, alpha | IL-2-RA | IL2-RA | TCGFR | IL-2R subunit alpha | p55 | TAC antigen
Host
Mouse
Reactivity
Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
OX-39
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Stimulated Rat T cells.
Specificity
Recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a 55kD type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla. *This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts. XXX recommend the use of MCA273EL for this purpose.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- Immunoprecipitation
- Flow Cytometry (1:10 - 1:50)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IL2RA / CD25
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, Flow Cytometry
Species:
Human
Applications:
Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
