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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL2RA / CD25 Antibody (clone MRC OX‑39, Azide‑free) LS‑C62774
Antibody
CD25 antibody LS-C62774 is an unconjugated mouse monoclonal antibody to rat CD25 (IL2RA). Validated for Flow and IHC.
Catalog
Size
Price
LS-C62774-1000
1000 µg (1 mg/ml)
Unavailable

Popular IL2RA / CD25 Products

Antibody
Species: Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunoprecipitation, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: Immunofluorescence, Flow Cytometry
Anti-CD25 antibody IHC of human plasma cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7396 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
IHC of paraffin-embedded rectum cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
IHC of CD25 on FFPE Tonsil tissue.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen

Product Description

CD25 antibody LS-C62774 is an unconjugated mouse monoclonal antibody to rat CD25 (IL2RA). Validated for Flow and IHC.
About IL2RA / CD25
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. P01589 NM_000417 NP_000408.1

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Specifications

Target
Rat IL2RA / CD25
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [MRC OX-39]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Flow Cytometry (0.1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IL2RA / CD25 antibody was raised against t blasts from a mixed lymphocyte reaction between purified CD4 positive T cells and irradiated spleens.
Specificity
Anti-rat CD25 (IL-2R) monoclonal antibody recognizes the smaller (alpha subunit) 55kD chain of the IL-2 receptor found on activated rat T cells, thymic dendritic cells but not resting lymphocytes. The antibody binds to the rat interleukin-2 receptor desig.
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, no preservative.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/24/2018

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