LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-IL2RA / CD25 Antibody (aa34-139, clone 1B5D12) LS-C191874

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL2RA / CD25 Antibodies

Anti-IL2RA / CD25 Antibody (clone IL2R.1, Azide-free) LS-C87934
Mouse Monoclonal [clone IL2R.1] (IgG1,k) to Human IL2RA / CD25
Human
Immunofluorescence, Flow Cytometry
Unconjugated, Azide-free
Anti-IL2RA / CD25 Antibody IHC-plus™ LS-B7396
Mouse Monoclonal (IgG2a,k) to Human IL2RA / CD25
Human
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1B5D12] (IgG1) to Human IL2RA / CD25
Human, Monkey, Mouse, Rat
IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated

Details

Human IL2RA / CD25
Mouse
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [1B5D12]
Unconjugated
Purified
Unmodified

Applications

  • IHC - Paraffin (1:200 - 1:1000)
  • ICC (1:200 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000)

Specificity and Use

IL2RA / CD25 antibody was raised against purified recombinant fragment of human IL2RA (AA: 34-139)
aa34-139
Human IL2RA / CD25

Packaging

PBS, 0.05% sodium azide, 0.05% protein stabilizer
Short term +4°C; Long term -20°C
For research use only.

About IL2RA / CD25

P01589 NM_000417 NP_000408.1

IL2RA Antibody, CD25 antigen Antibody, CD25 Antibody, IDDM10 Antibody, IL-2 receptor subunit alpha Antibody, IL2R Antibody, Interleukin 2 receptor, alpha Antibody, IL-2-RA Antibody, IL2-RA Antibody, TCGFR Antibody, IL-2R subunit alpha Antibody, p55 Antibody, TAC antigen Antibody

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded rectum cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded bladder cancer tissues using IL2RA mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence
Immunofluorescence of HeLa cells using IL2RA mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot
Western blot using IL2RA mouse monoclonal antibody against HeLa (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.

Western blot

Western blot
Western blot using IL2RA monoclonal antibody against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)

Western blot

Western blot
Western blot using IL2RA monoclonal antibody against HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
 

Requested From: United States
Date Requested: 12/4/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number