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Anti-IL27 Antibody LS-C154133

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL27 Antibodies

Anti-IL27 Antibody (N-Terminus) IHC-plus™ LS-B2565
Rabbit Polyclonal to Human IL27
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IL27 Antibody (Internal) IHC-plus™ LS-B2719
Rabbit Polyclonal to Human IL27
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL27 Antibody LS-C310763
Rabbit Polyclonal (IgG) to Human IL27
Human
Western blot, Immunoprecipitation
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse IL27
Mouse
Western blot, ELISA
Unconjugated

Details

Mouse IL27
Rabbit
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • Western blot (1:5000)
  • ELISA

Specificity and Use

IL27 antibody was raised against this purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL27/p28 protein.
This antibody is specific for mouse IL-27/p28 protein. A BLAST analysis was used to suggest cross-reactivity with IL-27A/p28 from mouse sources based on 100% homology with the immunizing sequence. Based on 90% or greater positive homology, there is a chance of cross-reactivity to rat. Cross-reactivity with IL-27 from other sources has not been determined. Cancer Immunology Research.
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23.6 kD in size corresponding to the mature mouse IL-27/p28 protein by western blotting in appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL27

Q8NEV9 NM_145659 NP_663634.2

IL27 Antibody, IL-27 Antibody, IL-27 p28 subunit Antibody, IL30 Antibody, Interleukin 27 Antibody, Interleukin-27 subunit alpha Antibody, IL27A Antibody, Interleukin 30 Antibody, IL-27-A Antibody, IL27p28 Antibody, IL-27 subunit alpha Antibody, IL-27A Antibody, IL27-A Antibody, p28 Antibody

Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells.

Western blot

Western blot
Anti-Mouse IL-27/p28 Antibody - Western Blot. Detection of recombinant IL27/p28 protein by anti-Mouse IL-27/p28 antibody. Recombinant mouse IL27/p28 was loaded on to an SDS-PAGE gel at 0.25 ug and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40000 in blocking buffer (MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
 

Requested From: United States
Date Requested: 12/7/2016

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