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Anti-IL27 Antibody (clone 3H12.F10) LS-C153844


Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular IL27 Antibodies

Anti-IL27 Antibody (N-Terminus) IHC-plus™ LS-B2565
Rabbit Polyclonal to Human IL27
Human, Mouse
IHC - Paraffin, ICC, Western blot
Immunohistochemistry Image
Anti-IL27 Antibody (Internal) IHC-plus™ LS-B2719
Rabbit Polyclonal to Human IL27
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-IL27 Antibody (C-Terminus) IHC-plus™ LS-B8257
Rabbit Polyclonal to Human IL27
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rat Monoclonal [clone 3H12.F10] (IgG) to Mouse IL27
Western blot, ELISA


Mouse IL27
Mouse (tested or 100% immunogen sequence identity)
IgG Monoclonal [3H12.F10]
Ion exchange chromatography


  • Western blot (1:1000)

Specificity and Use

IL27 antibody was raised against this Protein A purified monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E. coli) followed by hybridoma development.
This antibody is specific for mouse and rat p28 protein. Cross-reactivity with IL-27 from other sources has not been determined.
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL27

Q8NEV9 NM_145659 NP_663634.2

IL27 Antibody, IL-27 Antibody, IL-27 p28 subunit Antibody, IL30 Antibody, Interleukin 27 Antibody, Interleukin-27 subunit alpha Antibody, IL27A Antibody, Interleukin 30 Antibody, IL-27-A Antibody, IL27p28 Antibody, IL-27 subunit alpha Antibody, IL-27A Antibody, IL27-A Antibody, p28 Antibody

Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells.

Western blot

Western blot
Anti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 g) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-IL-27/p28 antibody diluted 1:1000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.

Flow Cytometry

Flow Cytometry
Mouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN? and 1 ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were harvested, washed, aliquoted 1x106 cells per sample, and fixed and permeabilized according to a standard protocol. Samples were stained with biotinylated primary anti-mouse p28 antibody at (0.1 - 10 ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25 ug/mL rat anti-mouse CD107b conjugated Alexa Fluor 647 and PHYCOERYTHRIN Conjugated secondary at 1:100 and analyzed by flow cytometry. Stimulated cells showed increase PE staining (horizontal axis) when compared with unstimulated cells.

Requested From: United States
Date Requested: 10/24/2016

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