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Anti-IL25 / IL17E Antibody (aa115-132, clone 68C1039.2) IHC-plus™ LS-B123

Note: This antibody replaces LS-C491

Ordering

Wt. Vol. Conc. Price
50 µg 100 µl 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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IL25 / IL17E Antibody LS-B123 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-IL-25 antibody IHC of human adrenal cortex.

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Mouse Monoclonal [clone 68C1039.2] (IgG1) to Human IL25 / IL17E
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human IL25 / IL17E
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [68C1039.2]
Protein G purified

Applications

IHC - Paraffin (10 µg/ml), Western blot (2 - 4 µg/ml)

Specificity and Use

IL25 / IL17E antibody was raised against synthetic peptide from human IL25 / IL-25.
aa115-132
A synthetic peptide of amino acids 115-132, obtained from the human IL-17E protein sequence, was used as immunogen
Immunohistochemistry: LS-B123 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody , followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B123 was determined to be 10 ug/ml.

Packaging

PBS containing 0.05% BSA and 0.05% sodium azide. Sourced in TCS.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About IL25 / IL17E

Q9H293 NM_022789 NP_073626.1

IL25 Antibody, Interleukin 17E Antibody, Interleukin 25 Antibody, Interleukin-25 Antibody, IL17E Antibody, IL-17E Antibody, IL-25 Antibody, Interleukin-17E Antibody

The IL17 proteins are a family of potent cytokines that act to induce proinflammatory responses. Studies have shown that IL17E binds strongly to IL17RB. Receptor binding of ligand has been shown to lead to the activation of nuclear factor kappa-B and production of IL8.

Immunohistochemistry

Immunohistochemistry
Anti-IL-25 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B123 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 4/25/2015

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