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| Catalog Number | Size | Price |
|---|---|---|
| LS-B123-50 | 50 µg (1 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human IL25 / IL17E Antibody (clone 68C1039.2, aa115‑132, IHC, WB) LS‑B123
IHC‑plus™ Monoclonal Mouse anti‑Human IL25 / IL17E Antibody (clone 68C1039.2, aa115‑132, IHC, WB) LS‑B123
Note: This antibody replaces LS-C491
Antibody:
IL25 / IL17E Mouse anti-Human Monoclonal (aa115-132) (68C1039.2) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
IL25 / IL17E Mouse anti-Human Monoclonal (aa115-132) (68C1039.2) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
IL17E antibody LS-B123 is an unconjugated mouse monoclonal antibody to IL17E (IL25) (aa115-132) from human. It is reactive with human and mouse. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human IL25 / IL17E
Synonyms
IL25 | Interleukin 17E | Interleukin 25 | Interleukin-25 | IL17E | IL-17E | IL-25 | Interleukin-17E
Host
Mouse
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
68C1039.2
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human IL25 / IL-25.
Epitope
aa115-132
Specificity
A synthetic peptide of amino acids 115-132, obtained from the human IL-17E protein sequence, was used as immunogen.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (2 - 4 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B123 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody , followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B123 was determined to be 10 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IL25 / IL17E
Validation
Anti-IL-25 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-IL-25 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ IL25 / IL17E Antibody (aa115-132, clone 68C1039.2) for IHC, WB/Western LS-B123 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Interleukin-25: a cytokine linking eosinophils and adaptive immunity in Churg-Strauss syndrome. Terrier B, Bièche I, Maisonobe T, Laurendeau I, Rosenzwajg M, Kahn JE, Diemert MC, Musset L, Vidaud M, Sène D, Costedoat-Chalumeau N, Le Thi-Huong D, Amoura Z, Klatzmann D, Cacoub P, Saadoun D. Blood. 2010 116:4523-31. (WB; Human)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
Western blot, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
