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Anti-IL22 Antibody IHC-plus™ LS-B7437

Note: This antibody replaces LS-C104291, LS-C187826

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL22 Antibodies

Anti-IL22 Antibody (aa40-67) LS-C83973
Rabbit Polyclonal to Human IL22
Human, Dog
IHC - Paraffin, Immunofluorescence, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL22 Antibody IHC-plus™ LS-B7437
Rabbit Polyclonal (IgG) to Human IL22
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human IL22
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated

Details

Human IL22
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (1.25 µg/ml)

Specificity and Use

IL22 antibody was raised against e.coli derived recombinant Human IL-22.
Human IL-22
Immunohistochemistry: LS-B7437 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-22 (50 ng/ml), a concentration of 1.25 ug/ml of this antibody is required. ELISA: To detect hIL-22 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-22 (LS-C104319) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-22. Western Blot: To detect hIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-22 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
50 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL22

Q9GZX6 NM_020525 NP_065386.1

IL22 Antibody, Cytokine Zcyto18 Antibody, ILTIF Antibody, Interleukin 22 Antibody, IL-22 Antibody, IL-D110 Antibody, TIFIL-23 Antibody, Zcyto18 Antibody, IL-TIF Antibody, Interleukin-22 Antibody

IL-22 a member of the IL-10 family of cytokines, which also includes IL-19, IL-20, IL-24, and IL-26, a class of potent mediators of cellular inflammatory responses. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells.

Immunohistochemistry

Immunohistochemistry
Anti-IIL-22 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7437 concentration 5 ug/ml.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL-22 antibody LS-B7437

Requested From: United States
Date Requested: 12/9/2016

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