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Anti-IL21 Antibody (aa24-152) LS-C154024


Wt. Vol. Conc. Price
100 µg - - Unavailable

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Rabbit Polyclonal (IgG) to Bovine IL21
Bovine, Horse, Pig
Western blot, ELISA


Bovine IL21
Bovine, Horse, Pig (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified


  • Western blot (1 µg/ml)

Specificity and Use

IL21 antibody was raised against recombinant protein produced in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein.
This antibody is specific for bovine IL-21 protein. A BLAST analysis was used to suggest cross-reactivity with IL-21 from bovine sources based on 100% homology with the immunizing sequence. Based on 92% homology, there is a chance of cross-reactivity to porcine IL- 21, 91% to horse IL-21, 89% to human and dog, 88% to macaque, 84% to platypus and Syrian hamster, 76-79% to mouse, 75% to rat, and 48% to chicken. Cross-reactivity with IL-21 from other sources has not been determined.
IL-21 is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes, and in HL-60 and THP-1 cell lines. This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.1 kD in size corresponding to bovine IL-21 protein by western blotting in the appropriate cell lysate or extract.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IL21

Q9HBE4 NM_021803 NP_068575.1

IL21 Antibody, Interleukin 21 Antibody, Interleukin-21 Antibody, Interleukin-21 isoform Antibody, Za11 Antibody, IL-21 Antibody

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG1 and IgG3 in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells.

Western blot

Western blot
Anti-bovine IL-21 Antibody - Western Blot. Western blot of anti-bovine IL-21 antibody shows detection of recombinant bovine IL-21 at 15.1kD (arrow) raised in yeast. Protein was purified and resolved by SDS-PAGE, transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with Anti-bovine IL-21. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody (.

Requested From: 
Date Requested: 4/24/2017

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