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Anti-IL2 Antibody LS-C104534

Note: This antibody replaces LS-C54323

Ordering

Wt. Vol. Conc. Price
500 µg - - $255
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL2 Antibodies

Anti-IL2 Antibody LS-C26969
Mouse Monoclonal (IgG2b) to Human IL2
Human
IHC, ELISA
Unconjugated
Anti-IL2 Antibody (Biotin) LS-C104555
Rabbit Polyclonal to Rat IL2
Rat
Western blot, ELISA, Neutralization
Biotin Conjugated
Western blot Image
Anti-IL2 Antibody IHC-plus™ LS-B7472
Goat Polyclonal to Human IL2
Human
IHC - Paraffin, Western blot, ELISA, Neutralization, Sandwich ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human IL2
Human
IHC - Paraffin, ELISA, Sandwich ELISA
Unconjugated

Details

Human IL2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (2.5 - 5 µg/ml)
  • ELISA (2 - 4 µg/ml)
  • Sandwich ELISA

Specificity and Use

IL2 antibody was raised against e. coli derived recombinant Human IL-2.
Human IL-2
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 2.5 ug/ml-5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant human IL-2 when used with biotinylated antigen affinity purified anti-human IL-2 (LS-C104522) as the detection antibody at a concentration of approximately 1-2 ug/ml. Western Blot: To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 1-2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 2-4 ng/lane, under reducing or non-reducing conditions.

Packaging

Lyophilized from PBS
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL2

P60568 NM_000586 NP_000577.2

IL2 Antibody, Aldesleukin Antibody, Interleukin 2 Antibody, Lymphokine Antibody, IL-2 Antibody, Interleukin-2 Antibody, TCGF Antibody, T cell growth factor Antibody, T-cell growth factor Antibody

IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene.

Immunohistochemistry

Immunohistochemistry
IHC of IL-2 antibody LS-C104534

Immunohistochemistry

Immunohistochemistry
IHC of IL-2 antibody LS-C104534

Western blot

Western blot
Western Blot (reducing) of IL-2 antibody LS-C104534

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL-2 antibody LS-C104534

Requested From: United States
Date Requested: 12/4/2016

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