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Catalog Number Size Price
LS-C313397-10 10 µg $318 
LS-C313397-100 100 µg $470 
IL18 Antibody - IL18 antibody. IHC(P): Human Tonsil Tissue.
IL18 Antibody - ICC analysis of IL18 using anti-IL18 antibody. IL18 was detected in immunocytochemical section of blood cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IL18 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen
IL18 Antibody - WB of IL18 antibody. All lanes: Anti-IL18 at 0.5ug/ml. WB: Recombinant Human IL18 Protein 0.5ng. Predicted bind size: 24KD. Observed bind size: 24KD.
IL18 Antibody - IL18 antibody. IHC(P): Human Tonsil Tissue.
IL18 Antibody - ICC analysis of IL18 using anti-IL18 antibody. IL18 was detected in immunocytochemical section of blood cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IL18 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen
IL18 Antibody - WB of IL18 antibody. All lanes: Anti-IL18 at 0.5ug/ml. WB: Recombinant Human IL18 Protein 0.5ng. Predicted bind size: 24KD. Observed bind size: 24KD.
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Polyclonal Rabbit anti‑Human IL18 Antibody (aa37‑193, IHC, WB) LS‑C313397

Polyclonal Rabbit anti‑Human IL18 Antibody (aa37‑193, IHC, WB) LS‑C313397

Note: This antibody replaces LS-C388670, LS-C388667
Antibody:
IL18 Rabbit anti-Human Polyclonal (aa37-193) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313397-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL18 Rabbit anti-Human Polyclonal (aa37-193) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL18 antibody LS-C313397 is an unconjugated rabbit polyclonal antibody to human IL18 (aa37-193). Validated for ELISA, IHC and WB. Cited in 2 publications.
Target
Human IL18
Synonyms
IL18 | IGIF | IL-1g | Interleukin-18 | IFN-gamma-inducing factor | IL-1 gamma | IL-18 | Iboctadekin | IL1F4 | Interleukin-1 gamma
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human IL-18 recombinant protein (Position: Y37-D193). Human IL-18 shares 66% and 64% amino acid (aa) sequences identity with mouse and rat IL-18, respectively.
Epitope
aa37-193
Applications
  • IHC
  • IHC - Paraffin (1 µg/ml)
  • Western blot (0.5 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC-P: HIER antigen retrieval with 10 mM sodium citrate buffer pH 6.0, 20 minutes at 99-100C is recommended.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL18
Q14116 NM_001562 NP_001553.1

LSBio Ratings

IL18 Antibody (aa37-193) for IHC, WB/Western, ELISA LS-C313397 has an LSBio Rating of
Publications (4.1)

Publications (2)

USP7 and USP47 deubiquitinases regulate NLRP3 inflammasome activation. Palazón-Riquelme P, Worboys JD, Green J, Valera A, Martín-Sánchez F, Pellegrini C, Brough D, López-Castejón G. EMBO reports. 2018 October;19:pii e44766.

Priming Is Dispensable for NLRP3 Inflammasome Activation in Human Monocytes In Vitro. Anna Gritsenko, Shi Yu, Fatima Martin-Sanchez, Ines Diaz-Del-Olmo, Eva-Maria Nichols, Daniel M Davis, David Brough, Gloria Lopez-Castejon. Frontiers in immunology. 2020 October;11:565924.

Customer Reviews (0)


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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/24/2024