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Anti-IL17F Antibody (clone 197301) LS-C70970

Ordering

Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml $485
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL17F Antibodies

Anti-IL17F Antibody (clone 197301) LS-C70970
Mouse Monoclonal [clone 197301] (IgG2b) to Human IL17F
Human
Flow Cytometry, ELISA
Unconjugated
Anti-IL17F Antibody (clone 4H1629) LS-C148702
Mouse Monoclonal [clone 4H1629] (IgG1,k) to Human IL17F
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-IL17F Antibody (aa31-141) LS-C308451
Mouse Monoclonal (IgG2a,k) to Human IL17F
Human
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 197301] (IgG2b) to Human IL17F
Human
Flow Cytometry, ELISA
Unconjugated

Details

Human IL17F
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [197301]
Unconjugated
Protein G purified
Unmodified

Applications

  • Flow Cytometry
  • ELISA

Specificity and Use

IL17F antibody was raised against e. coli-derived rhIL-17F corresponding to aa 31-163 according to Q96PD4.
Recognizes human IL-17F. In direct ELISAs and Western Blots this antibody does not cross-react with rhIL-17A, B, C, D, or E.
Suitable for use in ELISA and Flow Cytometry. The detection limit is ~25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry (Intracellular): 25 ug/ml. Add 10 ul to 1-5x10^5 cells in a total reaction volume not exceeding 200ul. For intracellular staining to detect IL-17F, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. This antibody was tested using PMA and Ca2+ ionomycin-treated PBMCs. ELISA (Direct): 0.5-1.0 ug/ml. The detection limit is ~1 ng/well.

Packaging

Lyophilized from PBS, 5% trehalose.
200 µl 200ul sterile PBS
Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
For research use only.

About IL17F

Q96PD4 Q96PD4

IL17F Antibody, CANDF6 Antibody, Cytokine ML-1 Antibody, IL-17F Antibody, Interleukin-17F Antibody, Interleukin 17F Antibody, Interleukin-24 Antibody, ML-1 Antibody, ML1 Antibody, Mutant IL-17F Antibody

Stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis.

Requested From: United States
Date Requested: 12/9/2016

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