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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL17A Antibody (Internal) LS‑C352378
IL17A antibody LS-C352378 is an unconjugated rabbit polyclonal antibody to IL17A from human, mouse and rat. Validated for WB.
Catalog
Size
Price
LS-C352378-100
100 µl (1 mg/ml)
$245

Popular IL17A Products

Anti-IL-17 antibody IHC of human t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7397 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-IL-17 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8323 dilution 5-10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Untreated (A) and PMA/ionomycin -stimulated (B) human PBMCs were stained using PE-conjugated mouse anti-human CD3 antibody and APC-conjugated antibody LS-C37030 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
Anti-Human IL-17A Western Blot Unreduced
Species: Human
Applications: Western blot, ELISA
Biotin Rabbit Anti-Rat IL-17A Antibody - Western Blot. Western Blot showing detection of Rat IL-17A. 50 ng of Rat IL-17A (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20°C, Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated LS-C154151 secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 15 kD molecular weight position expected for Rat IL-17A. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat, Mouse
Applications: IHC, Western blot, ELISA

Product Description

IL17A antibody LS-C352378 is an unconjugated rabbit polyclonal antibody to IL17A from human, mouse and rat. Validated for WB.
About IL17A
IL17A is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. Q16552 NM_002190 NP_002181.1

IL17A Antibody, CTLA-8 Antibody, CTLA8 Antibody, Interleukin-17A Antibody, Interleukin 17A Antibody, IL-17 Antibody, IL-17A Antibody, IL17 Antibody


Specifications

Target
Human IL17A
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:1000)
Immunogen
IL17A antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the central region of human IL-17.
Blocking Peptide
LS-E41646 - Lyophilized - 1 mg - $145.00
Immunizing peptide used to generate LS-C352378. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Recognizes endogenous levels of IL-17 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.

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Images

Western blot

Western blot analysis of IL-17 expression in A20 (A); THP1 (B); Raw264.7 (C); H9C2 (D) whole cell lysates.
Western blot analysis of IL-17 expression in A20 (A); THP1 (B); Raw264.7 (C); H9C2 (D) whole cell lysates.

Western blot

Western blot analysis of IL-17 expression in A20 (A); THP1 (B); Raw264.7 (C); H9C2 (D) whole cell lysates.
Western blot analysis of IL-17 expression in A20 (A); THP1 (B); Raw264.7 (C); H9C2 (D) whole cell lysates.

Western blot

Western blot analysis of IL-17 expression in A20 (A); THP1 (B); Raw264.7 (C); H9C2 (D) whole cell lysates.
Western blot analysis of IL-17 expression in A20 (A); THP1 (B); Raw264.7 (C); H9C2 (D) whole cell lysates.

Western blot

Western blot analysis of IL-17 expression in A20 (A); THP1 (B); Raw264.7 (C); H9C2 (D) whole cell lysates.
Western blot analysis of IL-17 expression in A20 (A); THP1 (B); Raw264.7 (C); H9C2 (D) whole cell lysates.

Requested From: United States
Date Requested: 9/26/2018

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