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Anti-IL17A Antibody LS-C104427

Note: This antibody replaces LS-C54097, LS-C37027

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable
100 µg - - Unavailable

Most Popular IL17A Antibodies

Anti-IL17A Antibody IHC-plus™ LS-B7397
Rabbit Polyclonal (IgG) to Human IL17A
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-IL17A Antibody (Biotin) LS-C154151
Rabbit Polyclonal (IgG) to Rat IL17A
Rat, Mouse
IHC, Western blot, ELISA
Biotin Conjugated
Western blot Image
Anti-IL17A Antibody (aa1-75, clone 4K5F6) IHC-plus™ LS-B8323
Mouse Monoclonal [clone 4K5F6] (IgG2b,k) to Human IL17A
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human IL17A
Human
IHC, Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Unconjugated

Details

Human IL17A
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (0.43 - 0.65 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

IL17A antibody was raised against e.coli derived recombinant Human IL-17A.
Human IL-17A
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 ug/ml-0.25 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-17A (50.00 ng/ml), a concentration of 0.43-0.65 ug/ml of this antibody is required. ELISA: To detect hIL-17A by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17A (LS-C104417) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Reconstitute in sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL17A

Q16552 NM_002190 NP_002181.1

IL17A Antibody, CTLA-8 Antibody, CTLA8 Antibody, Interleukin-17A Antibody, Interleukin 17A Antibody, IL-17 Antibody, IL-17A Antibody, IL17 Antibody

IL17A is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Immunohistochemistry

Immunohistochemistry
IHC of IL-17 antibody LS-C104427. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of IL-17 antibody LS-C104427. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of IL-17 antibody LS-C104427. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL-17 antibody LS-C104427. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 3/23/2017

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