(tested or 100% immunogen sequence identity)
IL17A antibody was raised against purified, E. coli-derived, recombinant human interleukin 17.
Suitable for use in Neutralization, Western Blot, Immunocytochemistry, Flow Cytometry, Direct ELISA. Neutralization: The ND50 is ~0.1-0.3 ug/ml in the presence of 25 ng/ml of rhIL-17, using the NHDF cell line. Immunocytochemistry: 2-15 ug/ml. Direct ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect human IL-17. The detection limit for rhIL-17 is approximately 0.2 ng/well. In this format, this antibody shows approximately 10% cross-reactivity with rmIL-17 and rhIL-17F and less than 1% cross-reactivity with rhIL-17B, rhIL-17C, rhIL-17D and rhIL-17E. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human IL-17. The detection limit for rhIL-17 is approximately 5 ng/lane under both non-reducing and reducing conditions. Flow Cytometry: Tested in PBMCs. For intracellular staining to detect IL-17, cells must be first fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled polyclonal antibodies can be visualized by a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome.
Lyophilized from PBS, pH 7.4, 5% Trehalose
Store at -20°C. Avoid freeze-thaw cycles.