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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL17A Antibody LS‑C37029
IL17A antibody LS-C37029 is an unconjugated goat polyclonal antibody to human IL17A. Validated for ELISA, Flow, ICC, Neut and WB.
Catalog
Size
Price
LS-C37029-100
100 µg (0.2 mg/ml)
$785
Description
IL17A antibody LS-C37029 is an unconjugated goat polyclonal antibody to human IL17A. Validated for ELISA, Flow, ICC, Neut and WB.
Target
Human IL17A
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (2 - 15 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • Flow Cytometry
  • ELISA (0.5 - 1 µg/ml)
  • Neutralization
Immunogen
IL17A antibody was raised against purified, E. coli-derived, recombinant human interleukin 17.
Specificity
Recognizes human IL-17.
Usage
Suitable for use in Neutralization, Western Blot, Immunocytochemistry, Flow Cytometry, Direct ELISA. Neutralization: The ND50 is ~0.1-0.3 ug/ml in the presence of 25 ng/ml of rhIL-17, using the NHDF cell line. Immunocytochemistry: 2-15 ug/ml. Direct ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect human IL-17. The detection limit for rhIL-17 is approximately 0.2 ng/well. In this format, this antibody shows approximately 10% cross-reactivity with rmIL-17 and rhIL-17F and less than 1% cross-reactivity with rhIL-17B, rhIL-17C, rhIL-17D and rhIL-17E. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human IL-17. The detection limit for rhIL-17 is approximately 5 ng/lane under both non-reducing and reducing conditions. Flow Cytometry: Tested in PBMCs. For intracellular staining to detect IL-17, cells must be first fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled polyclonal antibodies can be visualized by a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome.
Presentation
Lyophilized from PBS, pH 7.4, 5% Trehalose
Reconstitution
0.5 ml sterile PBS
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About IL17A
Q16552 NM_002190 NP_002181.1

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Requested From: United States
Date Requested: 12/10/2018
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