Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IL17A is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Anti-IL-17 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8323 dilution 5-10 ug/ml.
Western blot of IL-17A in 1) human, 2) mouse and 3) rat full-length recombinant IL-17A protein (100 ng/lane) using LS-B8323 at 0.2 ug/ml.
Intracellular flow cytometry of IL-17A in human lymphocytes stimulated with PMA (5 ng/ml), Ionomycin (1 mM), and Brefeldin A (1X) for 5 hrs, using LS-B8323 at 0.1 ug/10^6 cells (green represents isotype control, red represents IL-17A antibody). Goat anti-mouse IgG-FITC secondary antibody, was used for this test.
Requested From: United States
Date Requested: 10/23/2016